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Volume 69, Issue 6

Bacillus xiapuensis sp. nov., isolated from marine sediment Free

Abstract

A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China. Growth was observed at 10–30 °C (optimum, 25 °C), in 0–7.0 % NaCl (0 %) and at pH 6.0–11.0 (pH 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582 with the genus Bacillus , and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8 (97.6 %) and Bacillus ectoinformans (97.1 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain FJAT-46582 and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon. The DNA G+C content was 44.2 mol%. The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582 represents a novel Bacillus species, for which the name Bacillus xiapuensis sp. nov. is proposed. The type strain is FJAT-46582 (=JCM 33155=CCTCC AB 2017047).

  • Received:
  • Accepted:
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Keyword(s): ANI , Bacillus xiapuensis sp. nov. , coastal sediment , Fujian , isDDH , taxono-genomics and Xiapu
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2019-04-04
2024-04-20
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Bacillus xiapuensis sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 69, 1714 (2019); https://doi.org/10.1099/ijsem.0.003381
/content/journal/ijsem/10.1099/ijsem.0.003381
/content/journal/ijsem/10.1099/ijsem.0.003381
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