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Abstract

A novel aerobic, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain U0301, was isolated from a marine sediment sample in Weihai, China. This strain grew optimally at 33 °C, pH 7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. Strain U0301 shared pairwise 16S rRNA gene sequence similarities of 95.5, 95.4, 95.2 and 95.0 % to Parahaliea aestuarii S2-26, Halioglobus pacificus KCTC 23430, Halioglobus lutimaris HF004 and Halioglobus japonicus KCTC 23429, respectively. Phylogenetic analysis based on the 16S rRNA gene sequences demonstrated that U0301 formed a tight phylogenetic lineage with type strains of H. pacificus and H. japonicus . The percentage of conserved protein values of strain U0301 as compared with H. japonicus KCTC 23429and P arahaliea mediterranea 7SM29 were 62.5 and 58.0 %, respectively. The ANI values of strain U0301 with H. japonicus KCTC 23429 and P. mediterranea 7SM29 were 78.2 and 75.3 %, respectively. Both metrics of genome comparison suggested that strain U0301 showed higher homology with the genus Halioglobus than the genus Parahaliea . The strain contained ubiquinone 8 as the sole respiratory quinone. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and C17 : 1ω8c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 61.7 mol%. Considering the phenotypic characteristics, chemotaxonomic data and phylogenetic analysis comprehensively, strain U0301 should represent a novel species of the genus Halioglobus , for which the name of Halioglobus sediminis sp. nov. is proposed. The type strain is U0301 (=KCTC 62082=MCCC 1H00234).

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2019-03-25
2019-08-19
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