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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 6

Pigmentiphaga humi sp. nov., isolated from soil amended with humic acid Free

Abstract

A slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-318, was isolated from soil in a field located in Malvern, Alabama, USA. Phylogenetic analysis based on the 16S rRNA gene placed the strain within the genus Pigmentiphaga with highest 16S rRNA gene sequence similarity of 98.74 % and 98.67 % to the type strains of Pigmentiphaga kullae and Pigmentiphaga daeguensis , respectively. Sequence similarities to all other species of the genus were below 98.0 %. Results of the chemotaxonomic analysis, however, showed clear similarities to the genus Pigmentiphaga . The main cellular fatty acids of the strain were C16 : 0, C18 : 1 ω7c, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The major quinone was ubiquinone Q-8. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. In the polyamine pattern, putrescine and 2-hydroxyputrescine were predominant. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses, we propose a new species of the genus Pigmentiphaga , with the name Pigmentiphaga humi sp. nov. and strain IMT-318 (=LMG 30658=CIP 111626=CCM 8859) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Pigmentiphaga and Pigmentiphaga humi
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2019-04-08
2024-04-19
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Pigmentiphaga humi sp. nov., isolated from soil amended with humic acid
Int J Syst Evol Microbiol 69, 1573 (2019); https://doi.org/10.1099/ijsem.0.003355
/content/journal/ijsem/10.1099/ijsem.0.003355
/content/journal/ijsem/10.1099/ijsem.0.003355
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