1887

Abstract

A novel actinobacterial strain producing an antifungal substance was isolated from a sample of acidic mine area soil, and its taxonomic position was evaluated. The novel strain, designated TW1S1, formed white-grey aerial mycelium and yellow substrate mycelium on oatmeal agar. Growth occurred at 10–45 °C (optimum, 30 °C), pH 4–9 (pH 6–7) and in the presence of up to 8 % (w/v) NaCl. Melanin was produced on peptone–yeast extract–iron agar. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that the novel strain should be assigned to the genus Streptomyces , and the closest species was Streptomyces puniciscabiei S77 with 99.1 % sequence similarity, which was followed by Streptomyces durhamensis NRRL B-3309 (99.0 %), Streptomyces filipinensis NBRC 12860 (98.9 %) and Streptomyces yaanensis Z4 (98.7 %). The chemotaxonomic properties were consistent with those of Streptomyces . ll-Diaminopimelic acid was the diagnostic diamino acid, and alanine, glutamic acid and glycine were present in the peptidoglycan. The cell-wall hydrolysate also contained galactose, glucose, mannose and ribose. The predominant isoprenoid quinones were MK-9(H4) and MK-9(H6), the major polar lipids were phosphatidylglycerol and an unidentified phospholipid, and the main fatty acids were iso-C16 : 0 and anteiso-C15 : 0. However, strain TW1S1 could be distinguished from its neighbouring species by its phenotypic properties. In addition, the genome-based comparison with the closest species indicated that strain TW1S1 should be recognized as a separate species. The phylogenetic, phenotypic and chemotaxonomic as well as genomic evidence supported that TW1S1 represents a novel species of Streptomyces , for which the name Streptomyces fodineus sp. nov. is proposed (type strain, TW1S1 = KCTC 49013 = JCM 32404).

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2019-03-21
2019-08-22
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References

  1. Waksman SA, Henrici AT. The nomenclature and classification of the actinomycetes. J Bacteriol 1943;46:337–341[PubMed]
    [Google Scholar]
  2. Kämpfer P. Genus streptomyces. In Whitman WB. (editor) Bergey's Manual of Systematics of Archaea and Bacteria John Wiley & Sons; 2015; pp.1–414
    [Google Scholar]
  3. Bérdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 2012;65:385–395 [CrossRef][PubMed]
    [Google Scholar]
  4. Pochon J. Manuel technique d’analyse microbiologique du sol. Masson et Cie 1954
    [Google Scholar]
  5. Jones KL. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 1949;57:141[PubMed]
    [Google Scholar]
  6. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  7. Kelly K. Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washigton, DC: US Government Printing Office; 1964
    [Google Scholar]
  8. Tsukamura M. Numerical analysis of the relationship between Mycobacterium, Rhodochrous Group, and Nocardia by use of hypothetical median organisms. Int J Syst Bacteriol 1975;25:329–335 [CrossRef]
    [Google Scholar]
  9. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178:703 [CrossRef][PubMed]
    [Google Scholar]
  10. Tresner HD, Danga F. Hydrogen sulfide production by Streptomyces as a criterion for species differentiation. J Bacteriol 1958;76:239[PubMed]
    [Google Scholar]
  11. Kim MK, Kim TS, Joung Y, Han JH, Kim SB et al. nov., isolated from pine forest soil. Int J Syst Evol Microbiol 2016;66:3230–3234
    [Google Scholar]
  12. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014;64:689–691 [CrossRef][PubMed]
    [Google Scholar]
  13. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  14. Roh SG, Kim MK, Park S, Yun BR, Park J et al. Streptacidiphilus pinicola sp. nov., isolated from pine grove soil. Int J Syst Evol Microbiol 2018;68:3149–3155 [CrossRef][PubMed]
    [Google Scholar]
  15. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. Chemical Methods in Bacterial Systematics London: Academic Press; 1985; pp.267–287
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acid, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016;66:1100–1103 [CrossRef][PubMed]
    [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60–14 [CrossRef][PubMed]
    [Google Scholar]
  19. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  20. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR, Brenner D, Grimont P et al. Report of the ad hoc Committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  21. Zheng J, Zhang X, Xin Y, Han X, Ni S et al. Streptomyces yaanensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 2013;63:4719–4723 [CrossRef][PubMed]
    [Google Scholar]
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