RT Journal Article SR Electronic(1) A1 Jin, Long A1 Ko, So-Ra A1 Jin, Chun-Zhi A1 Jin, Feng-Jie A1 Li, Taihua A1 Ahn, Chi-Yong A1 Oh, Hee-Mock A1 Lee, Hyung-GwanYR 2019 T1 Description of novel members of the family Sphingomonadaceae: Aquisediminimonas profunda gen. nov., sp. nov., and Aquisediminimonas sediminicola sp. nov., isolated from freshwater sediment JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 8 SP 2179 OP 2186 DO https://doi.org/10.1099/ijsem.0.003347 PB Microbiology Society, SN 1466-5034, AB Two Gram-stain-negative bacterial strains, DS48-3T and CH68-4T, were isolated from freshwater sediment taken from the Daechung Reservoir, Republic of Korea. Cells of strains DS48-3T and CH68-4T were aerobic, non-motile, non-spore-forming and rod-shaped. Strain DS48-3T was isolated from a sediment surface sample at a depth of 48 m from the Daechung Reservoir and was most closely related to the genus Sphingopyxis according to 16S rRNA gene sequence analysis (94.5–95.9 % similarity). Strain CH68-4T was isolated from the very bottom of a 67-cm-long sediment core collected from Daechung Reservoir at a water depth of 17 m and was most closely related to the genus Sphingopyxis (16S rRNA gene sequence similarity of 93.7–95.0 %). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the two strains formed a separate lineage within the order Sphingomonadales showing similarity values below 95.9 % with their closest phylogenetic neighbours, and sharing 97.3 % similarity with each other. The combined genotypic and phenotypic data showed that strains DS48-3T and CH68-4T could be distinguished from all genera within the family Sphingomonadaceae and represented two distinct species of a novel genus, Aquisediminimonas profunda gen. nov., sp. nov. (type strain DS48-3T=KCTC 52068T=CCTCC AB 2018061T) and Aquisediminimonas sediminicola sp. nov. (type strain CH68-4T=KCTC 62205T=CCTCC AB 2018062T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003347