1887

Abstract

A novel Gram-stain-positive, catalase- and oxidase-positive, endospore-forming bacterium, designated GY 10110, was isolated from mangrove soil collected from Qinzhou, Guangxi province, China. Cells were aerobic, motile with peritrichous flagella and rod-shaped. The strain grew at 15–37 °C (optimum, 28 °C), at 0–3 %(w/v) NaCl (1 %) and at pH 6.0–9.0 (pH 7.0). The major fatty acids of strain GY 10110 were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. The predominant menaquinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, glycolipid, two unidentified aminophospholipids and three unidentified phospholipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GY 10110 was closely related to Falsibacillus pallidus CCTCC AB 207188 (98.0 % sequence similarity) and Bacillus oceanisediminis CGMCC 1.10115 (96.9 %), respectively. The G+C content of strain GY 10110 based on the whole genome sequence was 42.3 mol%. The novel strain showed an average nucleotide identity (ANI) value of 77.8 % and a digital DNA–DNA hybridization (dDDH) value of 15.6 % with Falsibacillus pallidus CCTCC AB 207188 based on draft genome sequences, followed by Bacillus oceanisediminis CGMCC 1.10115 with ANI and dDDH values of 75.2 and 12.8 %, respectively. The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analysis, showed that strain GY 10110 represents a novel species of the genus Falsibacillus , for which the name Falsibacillus albus sp. nov. is proposed. The type strain is GY 10110 (=CGMCC 1.13648=NBRC 113502).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003328
2019-03-06
2019-08-19
Loading full text...

Full text loading...

References

  1. Zhou Y, Xu J, Xu L, Tindall BJ. Falsibacillus pallidus to replace the homonym Bacillus pallidus Zhou et al. 2008. Int J Syst Evol Microbiol 2009;59:3176–3180 [CrossRef][PubMed]
    [Google Scholar]
  2. Zhou Y, Wei W, Che Q, Xu Y, Wang X et al. Bacillus pallidus sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2008;58:2850–2854 [CrossRef][PubMed]
    [Google Scholar]
  3. Gregersen T. Rapid method for distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 1978;5:123–127 [CrossRef]
    [Google Scholar]
  4. Smibert RM, Krieg NR. General characterization. In Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA et al. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981; pp.409–443
    [Google Scholar]
  5. Leifson E. Atlas of Bacterial Flagellation NY: Academic Press; 1960
    [Google Scholar]
  6. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005;55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  7. Choi JH, Seok JH, Cha JH, Cha CJ. Lysobacter panacisoli sp. nov., isolated from ginseng soil. Int J Syst Evol Microbiol 2014;64:2193–2197 [CrossRef][PubMed]
    [Google Scholar]
  8. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007
    [Google Scholar]
  9. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007;57:1424–1428 [CrossRef][PubMed]
    [Google Scholar]
  10. Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML et al. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci USA 1985;82:6955–6959 [CrossRef][PubMed]
    [Google Scholar]
  11. Niu L, Xiong M, Zhang J, Xiang Y, Song L et al. Bacillus camelliae sp. nov., isolated from Pu'er tea. Int J Syst Evol Microbiol 2018;68:564–569 [CrossRef][PubMed]
    [Google Scholar]
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  16. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  19. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics 2008;24:713–714 [CrossRef][PubMed]
    [Google Scholar]
  20. Li D, Liu CM, Luo R, Sadakane K, Lam TW. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics 2015;31:1674–1676 [CrossRef][PubMed]
    [Google Scholar]
  21. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  22. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29:319–322 [CrossRef]
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  24. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42:989–1005 [CrossRef]
    [Google Scholar]
  25. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207
    [Google Scholar]
  26. Nakagawa Y, Yamasato K. Phylogenetic diversity of the genus cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 1993;139:1155–1161 [CrossRef][PubMed]
    [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  28. Zhang J, Wang J, Fang C, Song F, Xin Y et al. Bacillus oceanisediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2010;60:2924–2929 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003328
Loading
/content/journal/ijsem/10.1099/ijsem.0.003328
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error