RT Journal Article SR Electronic(1) A1 Liu, Qing A1 Liu, Hong-Can A1 Zhou, Yu-Guang A1 Xin, Yu-HuaYR 2019 T1 Stenotrophobium rhamnosiphilum gen. nov., sp. nov., isolated from a glacier, proposal of Steroidobacteraceae fam. nov. in Nevskiales and emended description of the family Nevskiaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 5 SP 1404 OP 1410 DO https://doi.org/10.1099/ijsem.0.003327 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative strain, designated GT1R17T, was isolated from an ervoconite sample collected from Gawalong glacier in the Tibet Autonomous Region, PR China. Strain GT1R17T was catalase- and oxidase-positive, and grew optimally at 20–25°C and pH 7.0. The highest level of 16S rRNA gene sequence similarities were found to members of the genera Nevskia (92.27–93.15 %) and Hydrocarboniphaga (91.92–92.96 %). Phylogenetic analyses based on 16S rRNA gene sequences and genomic data revealed that the strain GT1R17T belonged to the family Nevskiaceae , but could not be assigned to any known genera. The genomic DNA G+C content was 54.4 mol%. The major fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1  ω7c and/or C18 : 1ω6c), C16 : 0, iso-C16 : 0 and summed feature 2 (C14 : 0 3OH and/or iso-C16 : 1 I). The polar lipids were phosphatidylglycerol, phosphatidylethanolamine and one unidentified lipid. The ubiquinone was Q-8. On the basis of the phenotypic, chemotaxonomic, genotypic and phylogenetic data, a novel species of a new genus,Stenotrophobiumrhamnosiphilum gen. nov., sp. nov. within the family Nevskiaceae , is proposed, with GT1R17T (=CGMCC 1.16137T=NBRC 113346T) as the type strain. In addition, phylogenetic analyses revealed that Steroidobacter and Povalibacter formed an independent clade in the order Nevskiales and were away from the families Nevskiaceae , Algiphilaceae and Salinisphaeraceae . Therefore, we propose to remove Steroidobacter and Povalibacter from the family Nevskiaceae and propose a new family Steroidobacteraceae in the order Nevskiales ., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003327