Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar

Mari Tohya; Shin Watanabe; Kanae Teramoto; Kohei Uechi; Tatsuya Tada; Kyoko Kuwahara-Arai; Takeshi Kinjo; Shiro Maeda; Isamu Nakasone; Ni Ni Zaw; San Mya; Khin Nyein Zan; Htay Htay Tin; Jiro Fujita; Teruo Kirikae

doi:10.1099/ijsem.0.003316

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Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar

Mari Tohya¹, Shin Watanabe², Kanae Teramoto³, Kohei Uechi⁴, Tatsuya Tada¹, Kyoko Kuwahara-Arai¹, Takeshi Kinjo⁴, Shiro Maeda⁵, Isamu Nakasone⁴, Ni Ni Zaw⁶, San Mya⁷, Khin Nyein Zan⁷, Htay Htay Tin⁷, Jiro Fujita⁴ and Teruo Kirikae¹
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Affiliations: ¹ 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan ² 2Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan ³ 3Koichi Tanaka Mass Spectrometry Research Laboratory, Kyoto, Japan ⁴ 4Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan ⁵ 5Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan ⁶ 6Clinical Pathology Section, Mandalay General Hospital, Mandalay, Myanmar ⁷ 7National Health Laboratory, Yangon, Myanmar
*Correspondence: Teruo Kirikae [email protected]
Published: 27 February 2019 https://doi.org/10.1099/ijsem.0.003316

Abstract

A novel Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, RYU5T, was isolated from a stool sample of an inpatient at a hospital in Okinawa, Japan. The optimal growth temperature of RYU5T was 30 °C. Phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA, rpoB, rpoD and gyrB genes, showed that RYU5T was a member of the Pseudomonas putida group and was located close to Pseudomonas monteilii and P. putida . Whole-genome comparisons, using average nucleotide identity and digital DNA–DNA hybridization, confirmed that strain RYU5T should be classified as a novel species of Pseudomonas . Phenotypic characterization tests showed that utilization of d-mannose, d-serine, l-arabinose and d-fructose could distinguish this strain from other related species of the genus Pseudomonas . Based on genetic and phenotypic evidence, strain RYU5T should be classified as a novel species, for which the name Pseudomonas asiatica sp. nov. is proposed. The type strain is RYU5T (=DSM 107182T, =JCM 32716T), with a DNA G+C content of 62.25 mol%.

Received: 26/06/2018
Accepted: 08/02/2019
Published Online: 27/02/2019

Keyword(s): human pathogen and Pseudomonas

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