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Abstract

A halophilic archaeon, strain F13-13, was isolated from a marine saltern located in Isla Cristina, Huelva, on the south-west coast of Spain. It was a Gram-stain-negative, motile and aerobic haloarchaeon. It grew at 28–50 °C (optimum, 37 °C), pH 6.0–8.5 (pH 7.5) and in 15–30 % (w/v) total salts (25 %). Phylogenetic analyses based on the 16S rRNA gene sequences showed that strain F13-13 is a member of the genus Halonotius , the most closely related species being Halonotius pteroides 1.15.5 (96.7 % sequence similarity). The 16S rRNA gene sequence similarity to species of other genera is lower than 93.4 %. Strain F13-13 was also found to be closely related to Halonotius pteroides 1.15.5 (91.4 %) on the basis of rpoB′ gene sequence analysis. The Genome-to-Genome Distance Calculator relatedness result between strain F13-13 and Halonotius pteroides CECT 7525 was 35 %, a value lower than the 70 % threshold accepted for species delineation. The average nucleotide identity values based on OrthoANI, ANIb and ANIm of strain F13-13 and Halonotius pteroides CECT 7525 were 88.7, 87.8 and 89.4 %, respectively, these values are also lower than the threshold accepted for species delineation. The DNA G+C content of this isolate was 61.2 mol%. The major lipids of strain F13-13 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulphated diglycosyl diether. Based on the phylogenetic, phenotypic, genotypic and chemotaxonomic characterization, we propose the placement of strain F13-13 as a new species within the genus Halonotius , with the name Halonotius aquaticus sp. nov. The type strain is F13-13 (=CECT 9386=IBRC-M 11204).

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2019-02-21
2019-10-22
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