1887

Abstract

Three Gram-stain-positive, catalase-negative, α-haemolytic, chain-forming and coccus-shaped microorganisms (strains Z15, Z1 and Z2) were isolated from feces of Tibetan antelopes collected from the Qinghai–Tibet plateau, PR China. The results of 16S rRNA gene sequence studies indicated that Z15 shared 94.5, 93.1 and 92.2 % similarity with DSM 102135, NUM 1615 and NUM 1529, respectively. and -based sequence analysis of our three novel isolates revealed interspecies divergence of 16.7 and 14.3 % from DSM 102135. The genomic DNA G+C content of Z15 is 42.48 mol%. Z15 has an average nucleotide identity (ANI) value of 81.19 % with DSM 102135 and a DNA–DNA relatedness value of less than 70 % in the DNA–DNA hybridization (isDDH) with other species of genus deposited in the GenBank database. A whole-genome phylogenetic tree based on 246 core genes of 78 genomes of members of the genus indicated that Z15 represents a member of genus but one well separated from the currently recognized species. Z15 contains Cω7 (25.5 %), Cω9 (19.6 %), C (17.5 %) and Cω9 (13.3 %) as its major cellular fatty acids. According to the morphological, biochemical and molecular phylogenetic features of the three novel isolates, they represent a novel species of the genus , and sp. nov. is thus proposed. The type strain is Z15 (=CGMCC 1.16529=DSM 106182).

Funding
This study was supported by the:
  • Agro-Industry R and D Special Fund of China (Award 2016YFC1201903)
  • National Natural Science Foundation of China (Award 81290340 and 81290345)
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
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2019-02-20
2021-10-19
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