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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 6

Croceicoccus ponticola sp. nov., a lipolytic bacterium isolated from seawater Free

Abstract

A lipolytic, Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GM-16, was isolated from seawater around Pohang in the Republic of Korea. Strain GM-16 grew optimally at 30 °C and in the presence of 1.0–2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GM-16 fell within the clade comprising the type strains of Croceicoccus species. Strain GM-16 exhibited the highest 16S rRNA gene sequence similarity (97.7 %) to the type strain of Croceicoccus pelagius and sequence similarities of 96.3–96.7 % to the type strains of the other Croceicoccus species. Strain GM-16 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids. The major polar lipids of strain GM-16 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and one unidentified glycolipid. The DNA G+C content of strain GM-16 was 62.6 mol%. The mean DNA–DNA relatedness value between strain GM-16 and C. pelagius DSM 101479 was 16 %. The average nucleotide identity values between strain GM-16 and the type strains of C.roceicoccus pelagius, C.roceicoccus marinus, C.roceicoccus naphthovorans and C.roceicoccus mobilis were 83.96–84.44 %. The phylogenetic and genetic data and differential phenotypic properties indicated that strain GM-16 is separated from recognized Croceicoccus species. On the basis of the data presented here, strain GM-16 is considered to represent a novel species of the genus Croceicoccus , for which the name Croceicoccus ponticola sp. nov. is proposed. The type strain is GM-16 (=KACC 19611=KCTC 62423=NBRC 113192).

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Keyword(s): Croceicoccus ponticola , novel species , polyphasic taxonomy and Seawater
© 2019 IUMS
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2019-04-16
2024-04-23
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Croceicoccus ponticola sp. nov., a lipolytic bacterium isolated from seawater
Int J Syst Evol Microbiol 69, 1551 (2019); https://doi.org/10.1099/ijsem.0.003301
/content/journal/ijsem/10.1099/ijsem.0.003301
/content/journal/ijsem/10.1099/ijsem.0.003301
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