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Volume 69, Issue 4

gen. nov., sp. nov. and proposal of fam. nov., representing new family in the order Free

Abstract

A novel bacterium with cells that were pinkish-cream-coloured, aerobic, rod-shaped, 0.62–1.00 µm wide and 2.3–3.3 µm long, designated as strain MEBiC09517, was isolated from Buksung-Po, a small port in Incheon, Republic of Korea. Strain MEBiC09517 had low 16S rRNA gene sequence similarity to validly reported strains; among them, SAORIC-476 displayed highest sequence similarity (89.9 %). Nevertheless, the novel strain shared a phylogenetic line with members of the genus , not the genus . Optimum growth conditions of strain MEBiC09517 were at 50–55 °C, pH 7 and in 2.0–4.0 % salt concentration. Strain MEBiC09517 was found to be an obligate marine bacterium that requires KCl, MgCl and CaCl as well as NaCl for growth. A phosphatidylethanolamine, a diphosphatidylglycerol, three glycolipids and four unidentified lipids were the strain's predominant polar lipid components. The fatty acid of the cell wall mainly consisted of carbons with 16 or 18 chain lengths such as C, C, C and summed feature 3 (Cω6 and/or Cω7). The predominant menaquinone was MK-7. The DNA G+C content is 68.65 mol%. Strain MEBiC09517 differs from genera of the order in terms of fatty acid composition, growth conditions, and range of carbon source utilization. Based on phylogenetic analysis using the strain's 16S rRNA gene sequence and results of physiological tests, strain MEBiC09517 (KCCM=43267, JCM=32374) is proposed as gen. nov., sp. nov. Additionally, the novel family fam. nov. based on phylogenetic analysis and physiological characteristics is suggested.

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Keyword(s): Rhodothermales , Roseithermus sacchariphilus gen. nov. sp. nov. and Salisaetaceae fam. nov
© 2019 IUMS
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2019-02-19
2024-04-19
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Roseithermus sacchariphilus gen. nov., sp. nov. and proposal of Salisaetaceae fam. nov., representing new family in the order Rhodothermales
Int J Syst Evol Microbiol 69, 1213 (2019); https://doi.org/10.1099/ijsem.0.003293
/content/journal/ijsem/10.1099/ijsem.0.003293
/content/journal/ijsem/10.1099/ijsem.0.003293
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