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Volume 69, Issue 4

sp. nov., isolated from paddy soil Free

Abstract

A Gram-stain-positive, motile, rod-shaped bacterial strain, YN-1, was isolated from a rice field in the town of Jietou, Yunnan Province, PR China. Colonies were circular, 1–2 mm in diameter, creamy white, with slightly irregular margins. The isolate grew optimally at 37 °C, pH 7.0 and with 1.0 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence similarity comparisons, YN-1 clustered together with other species of the genus and showed highest similarities with 0911MAR22V3 (98.0 %), LMG22167 (97.5 %), ' 10403023 (97.4 %) and ' P3516 (97.1 %). However, the DNA–DNA hybridization values between YN-1 and closely related strains of species of the genus were well below 47 %, indicating that they represent different taxa. The average nucleotide identity and the Genome-to-Genome Distance Calculator also revealed low relatedness (below 95 and 70 %, respectively) between YN-1 and type strains of closely related species of the genus . The DNA G+C content of the strain was 40 mol%. The major cellular fatty acids were iso-C, anteiso-C, and C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, three unidentified aminophospholipids and two other unidentified lipids. Physiological and biochemical test results were also different from those of the most closely related species. On the basis of the phenotypic, genetic and chemotaxonomic data, strain YN-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with strain YN-1 (=CCTCC AB 2017280=JCM 32973) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide , Bacillus aciditolerans , identity , paddy soil and polyphasic taxonomy
Funding
This study was supported by the:
  • Yunnan Provincial Technology Project of China Tobacco Corporation (Award No. 2018530000242017)
  • Guizhou Provincial Technology Project of China Tobacco Corporation (Award No. 201707)
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Bacillus aciditolerans sp. nov., isolated from paddy soil
Int J Syst Evol Microbiol 69, 1155 (2019); https://doi.org/10.1099/ijsem.0.003285
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