1887

Abstract

Strains 449 and 622 are both aerobic, Gram-stain-positive, short, rod-shaped bacilli that were recently isolated from the faeces of Tibetan antelopes on the Qinghai–Tibet Plateau in China. Their 16S rRNA gene sequences were most similar to those of ZD1-4(97.9–98.0 %) and CGMCC 1.11101(97.3–97.4 %). Phylogenetic analysis of the 16S rRNA gene sequences further suggested that strains 449 and 622 represent a new lineage within the genus The G+C content of strain 449 is 64.9 mol%. Optimal growth was achieved at pH 7.0 and 28 °C. Cells contained anteiso-C as the major cellular fatty acid, MK-10 and MK-11 as predominant menaquinones, diphosphatidylglycerol and phosphatidylglycerol as major polar lipids, and lysine as the diagnostic diamino acid. The DNA–DNA hybridization values of strains 449 and 622 were below the 70 % cut-off with respect to known strains of the genus . Based on these genotypic, phenotypic and biochemical characteristics, it seems rational to conclude that strains 449 and 622 belong to the genus and thus represent a novel species, for which the name sp. nov. is proposed. The type strain is 449 (=CGMCC 1.16372=DSM 106173).

Funding
This study was supported by the:
  • National Key R and D Program of China (Award 2016YFC1201903)
  • National Natural Science Foundation of China (Award National Natural Science Foundation of China)
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
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2019-02-14
2021-10-19
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