RT Journal Article SR Electronic(1) A1 Xiong, Qin A1 Wang, Dandan A1 Dong, Xiaoyan A1 Liu, Di A1 Liu, Yunpeng A1 Li, Pan A1 Wu, Gengwei A1 Luo, Yu A1 Zhang, Ruifu A1 Liu, Shengbing A1 Zhang, GuishanYR 2019 T1 Microbulbifer flavimaris sp. nov., a halophilic Gammaproteobacteria isolated from marine sediment of the Yellow Sea, China JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 4 SP 1135 OP 1141 DO https://doi.org/10.1099/ijsem.0.003279 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, aerobic, non-flagellated, non-motile bacterium, designated strain WRN-8T, was isolated from marine sediment of the Yellow Sea, China (36° 5′ 33′′ N, 121° 20′ 37′′ E). Colonies of strain WRN-8T were 0.2–0.3 µm wide, 2.1–2.8 µm long, catalase-positive and oxidase-positive. Colonies on marine agar solid media were circular, wet, smooth, light yellow and approximately 1.3 mm in diameter. Growth occurred optimally at 33–37 °C, pH 7.0–7.5 and in the presence of 2–4 % NaCl (w/v). Phylogenetic analysis of the 16S rRNA gene indicated that strain WRN-8T is a member of the genus Microbulbifer within the family Microbulbiferaceae , and the closest described neighbour in terms of 16S rRNA gene sequence identity is Microbulbifer aestuariivivens KCTC 52569T (98.1 %). The major respiratory quinone of strain WRN-8T is Q-8, its predominant fatty acids are iso-C15 : 0, iso-C17 : 0, C16 : 0, iso-C11 : 03-OH and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), and its major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, glycolipid, an unidentified phospholipid and an unidentified lipid. The draft genome obtained in this study was 3 643 020 bp, and the G+C content was 59.2 mol%. DNA–DNA hybridization (<46.3 %) and average nucleotide identity (<86.7 %) values between strain WRN-8T and the closest-related recognized Microbulbifer species confirmed the novelty of this new species. Therefore, we propose a novel species in the genus Microbulbifer to accommodate the novel isolate: Microbulbifer flavimaris sp. nov. (type strain WRN-8T=KCTC 42989T=ACCC 19926T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003279