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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 4

sp. nov., a halophilic Gammaproteobacteria isolated from marine sediment of the Yellow Sea, China Free

Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-motile bacterium, designated strain WRN-8, was isolated from marine sediment of the Yellow Sea, China (36° 5′ 33′′ N, 121° 20′ 37′′ E). Colonies of strain WRN-8 were 0.2–0.3 µm wide, 2.1–2.8 µm long, catalase-positive and oxidase-positive. Colonies on marine agar solid media were circular, wet, smooth, light yellow and approximately 1.3 mm in diameter. Growth occurred optimally at 33–37 °C, pH 7.0–7.5 and in the presence of 2–4 % NaCl (w/v). Phylogenetic analysis of the 16S rRNA gene indicated that strain WRN-8 is a member of the genus within the family , and the closest described neighbour in terms of 16S rRNA gene sequence identity is KCTC 52569 (98.1 %). The major respiratory quinone of strain WRN-8 is Q-8, its predominant fatty acids are iso-C, iso-C, C, iso-C3-OH and summed feature 3 (Cω6 and/or Cω7), and its major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, glycolipid, an unidentified phospholipid and an unidentified lipid. The draft genome obtained in this study was 3 643 020 bp, and the G+C content was 59.2 mol%. DNA–DNA hybridization (<46.3 %) and average nucleotide identity (<86.7 %) values between strain WRN-8 and the closest-related recognized species confirmed the novelty of this new species. Therefore, we propose a novel species in the genus to accommodate the novel isolate: sp. nov. (type strain WRN-8=KCTC 42989=ACCC 19926).

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  • Accepted:
  • Published Online:
Keyword(s): Gammaproteobacteria , marine sediment and Microbulbifer flavimaris
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2017YFD0201401)
  • National Natural Science Foundation of China (Award NSFC No. 31670113)
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2019-02-19
2024-04-18
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Microbulbifer flavimaris sp. nov., a halophilic Gammaproteobacteria isolated from marine sediment of the Yellow Sea, China
Int J Syst Evol Microbiol 69, 1135 (2019); https://doi.org/10.1099/ijsem.0.003279
/content/journal/ijsem/10.1099/ijsem.0.003279
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