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Volume 69, Issue 4

sp. nov., isolated from marine sponge Free

Abstract

A taxonomic study was carried out on strain HN-E23, which was isolated from sponge collected from Yangpu Bay, Hainan, China. Cells of strain HN-E23 were Gram-stain-negative, non-motile, orange-yellow-pigmented, short rods, that could grow at 10–40 °C (optimum, 28 °C), at pH 5–11 (optimun, pH 7) and in 0.5–12 % (w/v) NaCl (optimum, 3 %). This isolate was positive for oxidase, catalase, and the hydrolysis of aesculin, but negative for indole production and the reduction of nitrate. The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E23 formed a distinct phylogenetic lineage within the cluster comprising strains. Strain HN-E23 shared the highest 16S rRNA gene sequence similarity to JSSK-14 (97.2 %), followed by s21-N3 (96.6 %), KA37 (96.5 %) and RE35F/1 (96.4 %). The digital DNA–DNA hybridization (dDDH) and the average nucleotide identity (ANI) values between strain HN-E23 and JSSK-14 were 18.8 and 74.9 %, respectively. The dDDH and ANI values are below the standard cut-off criteria for delineation of bacterial species. The dominant fatty acids were summed feature 8 (Cω7ω6), C and summed feature 3 (Cω7ω6). The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid, an unidentified glycolipid and six unidentified lipids. The respiratory lipoquinone was identified as Q-10. The G+C content of the genomic DNA was 65.5 mol%. Based on the phenotypic and phylogenetic data, strain HN-E23 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain HN-E23 (=MCCC 1K03331=LMG 30457).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Erythrobacter spongiae sp. nov. , marine sponge and taxonomy
Funding
This study was supported by the:
  • Young and the Middle-Aged Teacher Education Scientific Research Project of Fujian Province (Award No. JT180644)
  • Scientific Research Project of Xiamen Southern Ocean Center (Award No. 17GYY008NF04)
  • Major Scientific Research Projects of Xiamen Medical College (Award XWJW201404-01)
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2019-02-12
2024-04-19
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References

  1. Shiba T, Simidu U. Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 1982; 32:211–217 [View Article]
     [Google Scholar]
  2. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–IN201 [View Article]
     [Google Scholar]
  3. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010; 2:117–134 [View Article][PubMed]
     [Google Scholar]
  4. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article][PubMed]
     [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  6. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  7. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  8. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the Ad Hoc Committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  9. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  10. Høvik Hansen G, Sørheim R. Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 1991; 13:231–241 [View Article]
     [Google Scholar]
  11. Smibert RM. Phenotypic characterization. Methods for General and Molecular Bacteriology 1994
     [Google Scholar]
  12. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note # 101. 1990
     [Google Scholar]
  13. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  14. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  15. Groth I, Schumann P, Weiss N, Martin K, Rainey FA. Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 1996; 46:234–239 [View Article][PubMed]
     [Google Scholar]
  16. Park S, Jung YT, Choi SJ, Yoon JH. Erythrobacter aquimixticola sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2017; 67:2964–2969 [View Article][PubMed]
     [Google Scholar]
  17. Zhuang L, Liu Y, Wang L, Wang W, Shao Z. Erythrobacter atlanticus sp. nov., a bacterium from ocean sediment able to degrade polycyclic aromatic hydrocarbons. Int J Syst Evol Microbiol 2015; 65:3714–3719 [View Article][PubMed]
     [Google Scholar]
  18. Lei X, Zhang H, Chen Y, Li Y, Chen Z et al. Erythrobacter luteus sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2015; 65:2472–2478 [View Article][PubMed]
     [Google Scholar]
  19. Jung YT, Park S, Oh TK, Yoon JH. Erythrobacter marinus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62:2050–2055 [View Article][PubMed]
     [Google Scholar]
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Erythrobacter spongiae sp. nov., isolated from marine sponge
Int J Syst Evol Microbiol 69, 1111 (2019); https://doi.org/10.1099/ijsem.0.003278
/content/journal/ijsem/10.1099/ijsem.0.003278
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