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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 69, Issue 4

sp. nov., a novel actinomycete isolated from cadmium-contaminated soil Free

Abstract

A novel strain, ZFG47, isolated from a cadmium-contaminated soil sample, was taxonomically studied in detail. Strain ZFG47 formed long, flexuous spiral spore chains consisting of elliptoid spores with spiny surfaces. The cell-wall hydrolysates contained -diaminopimelic acid as the diagnostic diamino acid. The major menaquinones consisted of MK-9(H2), MK-9(H4) and MK-9(H8). The major polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were iso-C, C and anteiso-C. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus , showing the highest sequence similarity to VK-A60 (98.7 %). However, the digital DNA–DNA hybridization value, the average nucleotide identity value and the MLSA evolutionary distance between this strain and VK-A60 showed that it belonged to a distinct species. Furthermore, the novel isolate could be distinctly differentiated from VK-A60 by morphological, physiological and biochemical characteristics. On the basis of the evidence from this polyphasic study, it is concluded that strain ZFG47 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain ZFG47 (CICC 11050=JCM 32897) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cadmium-contaminated soil , polyphasic taxonomy and Streptomtces cadmiisoli sp. nov.
Funding
This study was supported by the:
  • Education Department of Hunan Province (Award 16K032)
  • National Key R andD Program of China (Award 2017YFE011760)
© 2019 IUMS
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2019-02-04
2025-01-18
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Streptomyces cadmiisoli sp. nov., a novel actinomycete isolated from cadmium-contaminated soil
Int J Syst Evol Microbiol 69, 1024 (2019); https://doi.org/10.1099/ijsem.0.003262
/content/journal/ijsem/10.1099/ijsem.0.003262
/content/journal/ijsem/10.1099/ijsem.0.003262
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