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Abstract

A novel Streptomyces strain, ZFG47, isolated from a cadmium-contaminated soil sample, was taxonomically studied in detail. Strain ZFG47 formed long, flexuous spiral spore chains consisting of elliptoid spores with spiny surfaces. The cell-wall hydrolysates contained ll-diaminopimelic acid as the diagnostic diamino acid. The major menaquinones consisted of MK-9(H2), MK-9(H4) and MK-9(H8). The major polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were iso-C16 : 0, C16 : 0 and anteiso-C15 : 0. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces , showing the highest sequence similarity to Streptomyces koyangensis VK-A60 (98.7 %). However, the digital DNA–DNA hybridization value, the average nucleotide identity value and the MLSA evolutionary distance between this strain and S. koyangensis VK-A60 showed that it belonged to a distinct species. Furthermore, the novel isolate could be distinctly differentiated from S. koyangensis VK-A60 by morphological, physiological and biochemical characteristics. On the basis of the evidence from this polyphasic study, it is concluded that strain ZFG47 represents a novel species of the genus Streptomyces , for which the name Streptomyces cadmiisoli sp. nov. is proposed, with strain ZFG47 (CICC 11050=JCM 32897) as the type strain.

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2019-02-04
2019-10-21
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