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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 5

Nocardioides ferulae sp. nov., isolated from root of an endangered medicinal plant Ferula songorica Pall. ex Spreng Free

Abstract

A novel endophytic bacterium, designated strain SZ4R5S7, was isolated from surface-sterilized root of an endangered medicinal plant (Ferula songorica Pall. ex Spreng) collected from Xinjiang, north-west China. The taxonomic position of the strain was investigated by using a polyphasic approach. The strain was found to be aerobic, Gram-stain-positive, oxidase-negative and catalase-positive, short rods and non-motile. Strain SZ4R5S7 grew at 4–37 °C (optimum, 28 °C), pH 5.0–9.0 (pH 6.0–8.0) and in the presence of 0–4 % (w/v) NaCl. The polar lipids detected for strain SZ4R5S7 were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified lipids. The predominant menaquinone of strain SZ4R5S7 was MK-8(H4), and the major fatty acids were iso-C16 : 0 and anteiso-C14 : 0. The DNA G+C content was determined to be 72.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SZ4R5S7 belongs to the genus Nocardioides and showed highest levels of 16S rRNA gene sequence similarity to members of the strain Nocardioides kribbensis KSL-2 (97.8 %). On the basis of phenotypic, genotypic and phylogenetic data, strain SZ4R5S7 represents a novel species in the genus Nocardioides, for which the name Nocardioides ferulae sp. nov. is proposed and the type strain is SZ4R5S7 (=CGMCC 4.7456=KCTC 39994).

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Keyword(s): Ferula songorica , Nocardioides ferulae sp. nov. and polyphasic taxonomy
© 2019 IUMS
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2019-02-08
2024-04-18
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Nocardioides ferulae sp. nov., isolated from root of an endangered medicinal plant Ferula songorica Pall. ex Spreng
Int J Syst Evol Microbiol 69, 1253 (2019); https://doi.org/10.1099/ijsem.0.003261
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