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Volume 69, Issue 3

Marinomonas shanghaiensis sp. nov., isolated from the junction between an ocean and a freshwater lake Free

Abstract

A Gram-stain-negative, motile with single polar flagellum, rod-shaped bacterium, designated as strain DSL-35, was isolated from the location where the ocean and Dishui lake meet at Shanghai on the East China Sea and characterized phylogenetically and phenotypically. Optimal growth occurred at 35 °C (range, 4–40 °C), pH 8 pH 5–11) and with 3–4 % (w/v) NaCl (0–12 %). Phylogenetic analysis based on its 16S rRNA gene sequence showed that strain DSL-35 was related to members of the genus Marinomonas and shared the highest sequence identities with Marinomonas arctica 328 (98.0 %), Marinomonas hwangdonensis HDW-15 (97.5 %) and Marinomonas rhizomae IVIA-Po-145 (97.2 %). The 16S rRNA gene sequence identities between strain DSL-35 and other members of the genus Marinomonas were below 96.8 %. The digital DNA–DNA hybridization values between strain DSL-35 and the three type strains, Marinomonas . arctica 328, M. rhizomae HDW-15 and M. rhizomae IVIA-Po-145, were 30.9±2.4 %, 21.7±2.2% and 22±2.3 %, respectively. The average nucleotide identity values between strain DSL-35 and the three type strains were 87.6 %, 84.6 and 84.2 %, respectively. The predominant ubiquinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The predominant cellular fatty acids of strain DSL-35 were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 40.0 %), C16 : 0 (22.5 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 11.2 %), summed feature 2 (C14 : 0 3-OH and/or iso I C16 : 1; 7.2 %), C14 : 0 (6.8 %) and C12 : 0 (5.2 %). The G+C content of the genomic DNA was 44.5 mol%. The combined genotypic and phenotypic data indicated that strain DSL-35 represents a novel species of the genus Marinomonas , for which the name Marinomonas shanghaiensis sp. nov. is proposed, with the type strain DSL-35 (=KCTC 62646=MCCC 1K03535).

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Keyword(s): Dishui Lake , Marinomonas shanghaiensis and proteobacteria
© Microbiology Society
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2019-01-28
2024-04-18
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Marinomonas shanghaiensis sp. nov., isolated from the junction between an ocean and a freshwater lake
Int J Syst Evol Microbiol 69, 805 (2019); https://doi.org/10.1099/ijsem.0.003241
/content/journal/ijsem/10.1099/ijsem.0.003241
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