1887

Abstract

A sulfur-oxidizing, filamentous, gliding micro-organism, strain D3, was isolated from a sulfidic spring in Goryachy Klyuch, Krasnodar, Russia. The cell walls were Gram-negative. The new isolate was a microaerophilic facultative anaerobe and an obligate chemolithoautotroph. The pH range for growth was pH 6.8–7.6, with an optimum at pH 7.2. The temperature range for growth was 10–46 °C, with an optimum at 32 °C. The G+C content of DNA was 42.1 mol%. Phylogenetic analysis of the 16S rRNA gene showed that strain D3 belongs to the family Beggiatoaceae , order Thiotrichales and was distantly related to the genera of the family Beggiatoaceae (86–88 % sequence similarity). The major respiratory quinone was ubiquinone-6. Major fatty acids were C18:1 ω7 (37.6 %), C16 : 0 (34.7 %) and C16: 1 ω7 (27.7 %). On the basis of its physiological properties and the results of phylogenetic analysis, strain D3 is considered to represent a novel species of a new genus, for which the name Thioflexithrix psekupsensis gen. nov., sp. nov. is proposed. The type strain is D3 (=KCTC 62399=UNIQEM U981).

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2019-01-18
2024-12-07
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References

  1. Salman V, Amann R, Girnth AC, Polerecky L, Bailey JV et al. A single-cell sequencing approach to the classification of large, vacuolated sulfur bacteria. Syst Appl Microbiol 2011; 34:243–259 [View Article][PubMed]
    [Google Scholar]
  2. Dubinina G, Savvichev A, Orlova M, Gavrish E, Verbarg S et al. Beggiatoa leptomitoformis sp. nov., the first freshwater member of the genus capable of chemolithoautotrophic growth. Int J Syst Evol Microbiol 2017; 67:197–204 [View Article][PubMed]
    [Google Scholar]
  3. Ahmad A, Kalanetra KM, Nelson DC. Cultivated Beggiatoa spp. define the phylogenetic root of morphologically diverse, noncultured, vacuolate sulfur bacteria. Can J Microbiol 2006; 52:591–598 [View Article][PubMed]
    [Google Scholar]
  4. De Albuquerque JP, Keim CN, Lins U. Comparative analysis of Beggiatoa from hypersaline and marine environments. Micron 2010; 41:507–517 [View Article][PubMed]
    [Google Scholar]
  5. Hinck S, Neu TR, Lavik G, Mussmann M, De Beer D et al. Physiological adaptation of a nitrate-storing Beggiatoa sp. to diel cycling in a phototrophic hypersaline mat. Appl Environ Microbiol 2007; 73:7013–7022 [View Article][PubMed]
    [Google Scholar]
  6. Teske A, Ramsing NB, Küver J, Fossing H. Phylogeny of Thioploca and related filamentous sulfide-oxidizing bacteria. Syst Appl Microbiol 1995; 18:517–526 [View Article]
    [Google Scholar]
  7. Pfennig N, Lippert KD. Über das vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch Microbiol 1966; 55:425–432
    [Google Scholar]
  8. Kuznetsov SI, Dubinina GA. Methods of Investigation of Aqueous Microorganisms Moscow: Nauka; 1989 pp. 285
    [Google Scholar]
  9. Reznikov AA, Mulikovskaya EP, Vyu S. Metody Analiza Prirodnykh vod (Methods for analysis of natural waters). Moscow Gosgeoltekhizdat; 1970
  10. Stewart WD, Fitzgerald GP, Burris RH. Acetylene reduction by nitrogen-fixing blue-green algae. Arch Mikrobiol 1968; 62:336–348 [View Article][PubMed]
    [Google Scholar]
  11. Frolo EN, Belousova EV, Lavrinenko KS, Dubinina GA, Grabovich MIu. [The detection of Azospirillum thiophilum ability for lithotrophy during oxidation of reduced sulfur compounds]. Mikrobiologiia 2013; 82:274–283[PubMed]
    [Google Scholar]
  12. Cammack R, Fernandez VM, Hatchikian EC. Nickel-iron hydrogenase. Meth Enzymol 1994; 243:43–68
    [Google Scholar]
  13. Yu L, Wolin MJ. Hydrogenase measurement with photochemically reduced methyl viologen. J Bacteriol 1969; 98:51–55[PubMed]
    [Google Scholar]
  14. Slobodkin AI, Slobodkina GB, Panteleeva AN, Chernyh NA, Novikov AA et al. Dissulfurimicrobium hydrothermale gen. nov., sp. nov., a thermophilic, autotrophic, sulfur-disproportionating deltaproteobacterium isolated from a hydrothermal pond. Int J Syst Evol Microbiol 2016; 66:1022–1026 [View Article][PubMed]
    [Google Scholar]
  15. Fomenkov A, Vincze T, Grabovich MY, Dubinina G, Orlova M et al. Whole-genome sequence and methylome analysis of the freshwater colorless sulfur bacterium Thioflexothrix psekupsii D3. Genome Announc 2017; 5:e0090400917 [View Article][PubMed]
    [Google Scholar]
  16. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35:1547–1549 [View Article][PubMed]
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  18. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  19. Zuckerkandl E, Pauling L. Molecules as documents of evolutionary history. J Theor Biol 1965; 8:357–366 [View Article][PubMed]
    [Google Scholar]
  20. Le SQ, Gascuel O. An improved general amino acid replacement matrix. Mol Biol Evol 2008; 25:1307–1320 [View Article][PubMed]
    [Google Scholar]
  21. Friedrich CG, Rother D, Bardischewsky F, Quentmeier A, Fischer J. Oxidation of reduced inorganic sulfur compounds by bacteria: emergence of a common mechanism?. Appl Environ Microbiol 2001; 67:2873–2882 [View Article][PubMed]
    [Google Scholar]
  22. Weissgerber T, Dobler N, Polen T, Latus J, Stockdreher Y et al. Genome-wide transcriptional profiling of the purple sulfur bacterium Allochromatium vinosum DSM 180T during growth on different reduced sulfur compounds. J Bacteriol 2013; 195:4231–4245 [View Article][PubMed]
    [Google Scholar]
  23. Tamoi M, Ishikawa T, Takeda T, Shigeoka S. Molecular characterization and resistance to hydrogen peroxide of two fructose-1,6-bisphosphatases from Synechococcus PCC 7942. Arch Biochem Biophys 1996; 334:27–36 [View Article][PubMed]
    [Google Scholar]
  24. Badger MR, Bek EJ. Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle. J Exp Bot 2008; 59:1525–1541 [View Article][PubMed]
    [Google Scholar]
  25. Orlova MV, Shatsky ND, Belousova EV, Grabovich MY. The ability of freshwater filamentous sulfur bacteria from the family Beggiatoaceae to assimilate molecular nitrogen: molecular detection and expression of nifH - the marker gene of nitrogen fixation. Sorption and Chromatographic Processes 2016; 16:280–285
    [Google Scholar]
  26. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [View Article][PubMed]
    [Google Scholar]
  27. Mezzino MJ, Strohl WR, Larkin JM. Characterization of Beggiatoa alba. Arch Microbiol 1984; 137:139–144 [View Article]
    [Google Scholar]
  28. Kobayashi S, Shibata H. Metabolic characteristics of Beggiatoa alba in thiosulfate medium and porcine colon contents. Nihon Chikusan Gakkaiho 1999; 70:349–355 [View Article]
    [Google Scholar]
  29. Kreutzmann AC. Electron Donors and Acceptors for Members of the Family Beggiatoaceae 2013 The University of Bremen Ph.D thesis
    [Google Scholar]
  30. Kappler U, Maher MJ. The bacterial SoxAX cytochromes. Cell Mol Life Sci 2013; 70:977–992 [View Article][PubMed]
    [Google Scholar]
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