An arsenic-resistant strain, CB3T, was isolated from arsenic-rich aquifers at the Jianghan Plain in Hubei, China. Phylogenetic and biochemical analysis suggested that it should represent a new species of the genus Pseudaminobacter in the family Phyllobacteriaceae. The 16S rRNA gene of CB3T shared the highest sequence similarities to those of the type strains Pseudaminobacter defluvii THI 051T (97.8 % identity) and Pseudaminobacter salicylatoxidans BN12T (97.4 %). The DNA–DNA relatedness values of CB3T with respect to strains belonging to the genus Pseudaminobacter were less than 70 %. The fatty acid profile of CB3T consisted of C16 : 0, cyclo-C19 : 0ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as major components. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and diphosphatidylglycerol. The DNA G+C content was 61.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CB3T was distinct from previously described Pseudaminobacter species. Therefore, we propose that strain CB3T represents a novel species of the genus Pseudaminobacter, Pseudaminobacterarsenicus sp. nov., strain CB3T (=CCTCC AB2016116T=KCTC 52625T) is designated as the type strain.
KampferP, MullerC, MauM, NeefA, AulingG et al. Description of Pseudaminobacter gen nov. with two new species P. salicylatoxidans sp. nov. and P. defluvii sp. nov. Intl J Syst Bacteriol1999; 149:887–897
ChenX, ZengXC, WangJ, DengY, MaT et al. Microbial communities involved in arsenic mobilization and release from the deep sediments into groundwater in Jianghan plain, Central China. Sci Total Environ2017; 579:989–999 [View Article][PubMed]
ZengXC, GjE, WangJ, WangN, ChenX et al. Functional characterization and unique diversity of genes and microorganisms involved in arsenite oxidation from the tailings of a realgar mine. Appl Environ Microb201602190–16
ArturssonV, JanssonJK. Use of bromodeoxyuridine immunocapture to identify active bacteria associated with arbuscular mycorrhizal hyphae. Appl Environ Microbiol2003; 69:6208–6215 [View Article][PubMed]
YoonSH, HaSM, KwonS, LimJ, KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article][PubMed]
KimM, OhHS, ParkSC, ChunJ. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol2014; 64:346–351 [View Article][PubMed]
KhanIU, HabibN, AsemMD, SalamN, XiaoM et al.Aquabacterium tepidiphilum sp. nov., a moderately thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol2018 [View Article][PubMed]
MinnikinDE, O'DonnellAG, GoodfellowM, AldersonG, AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]
MuY, ZhouL, ZengXC, LiuL, PanY et al.Arsenicitalea aurantiaca gen. nov. sp. nov. a new member of the family hyphomicrobiaceae, isolated from the high-arsenic sediment in Jianghan plain. Int J Syst Evol Microbiol2016; 66:1–7
LiR, ZhuH, RuanJ, QianW, FangX et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res2010; 20:265–272 [View Article][PubMed]
RichterM, Rosselló-MóraR, Oliver GlöcknerF, PepliesJ. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics2016; 32:929–931 [View Article][PubMed]
GorisJ, KonstantinidisKT, KlappenbachJA, CoenyeT, VandammeP et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol2007; 57:81–91 [View Article][PubMed]
GrahamPH, SadowskyMJ, KeyserHH, BarnetYM, BradleyRS et al. Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int J Syst Bacteriol1991; 41:582–587 [View Article]
WilsonK. Preparation of genomic DNA from bacteria. In AusubelFM, BrentR, KingstonRE, MooreDD, SeidmanJG et al. (editors) Current Protocols in Molecular Biology New York: Green Publishing & Wiley- Interscience; 1987 pp. 2.4.1–2.4.2
CleenwerckI, VandemeulebroeckeK, JanssensD, SwingsJ. Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol2002; 52:1551 [View Article][PubMed]
WayneLG, BrennerDJ, ColwellRR, GrimontPAD, KandlerO et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol1987; 37:463–464
StackebrandtE, GoebelBM. Taxonomic Note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol1994; 44:846–849 [View Article]