1887

Abstract

A novel Gram-positive, non-motile, non-spore-forming and aerobic bacterium, designated strain VA37-3, was isolated from a marine sediment sample collected at 19.2 m water depth from Valparaíso bay, Chile. Strain VA37-3 exhibits 97.6 % 16S rRNA gene sequence similarity to Corynebacterium marinum D7015, 96.4 % to Corynebacterium humireducens MFC-5 and 96 % to Corynebacterium testudinoris M935/96/4; and a rpoB gene sequence similarity of 85.1 % to Corynebacterium pollutisoli VMS11, both analyses suggesting that strain VA37-3 represents a novel species of Corynebacterium . Physiological testing indicated that strain VA37-3 requires artificial sea water or sodium-supplemented media for growth, representing the first obligate marine actinomycete of the genus Corynebacterium . The genome of the proposed new species, along with the type strains of its most closely related species were sequenced and characterized. In silico genome-based similarity analyses revealed an ANIb of 72.8 % ( C. marinum D7015), ANIm of 85.0 % ( Corynebacterium mustelae DSM 45274), tetra of 0.90 ( Corynebacterium callunae DSM 20147) and ggdc of 24.7 % ( Corynebacterium kutscheri DSM 20755) when compared with the closest related strains. The genomic DNA G+C content of strain VA37-3 was 57.0 %. Chemotaxonomic assessment of strain VN6-2 showed the major fatty acids were C18 : 1ω9c and C16 : 0. Menaquinones predominantly consisted of MK-8(II-H2). Polar lipids consisted of diphosphatidylglycerol, glycolipids, phosphatidylglycerol, phosphoglycolipid and phosphatidylinositol. Mycolic acids also were present. Overall, the results from phylogenetic, phenotypic and genomic analyses confirmed that strain VA37-3 represents a novel species of the genus Corynebacterium , for which the name Corynebacterium alimapuense sp. nov. is proposed, with VA37-3 as the type strain (=CCUG 69366=NCIMB 15118).

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2019-01-28
2024-03-28
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