Mycoplasma hafezii sp. nov., isolated from the trachea of a peregrine falcon (Falco peregrinus) Free

Abstract

Mycoplasma species are well known pathogens in avian medicine, especially in poultry. However, several Mycoplasma species have been regularly found in the respiratory tract of birds of prey which seem to be commensals in these bird species. In previous studies, an unknown Mycoplasma species which caused false positive results in a Mycoplasma meleagridis -specific PCR, was isolated from a tracheal swab of a clinically healthy, captive, adult peregrine falcon (Falco peregrinus). The isolate appeared in typical fried-egg-shaped colonies on SP4 agar plates and was dependent on sterol for growth. Acid was produced from glucose, but no arginine or urea was hydrolysed. The temperature range for growth was 28–44 °C, with an optimum at 37 °C. Strain M26 was serologically distinct from all species of the genus Mycoplasma with 16S rRNA gene sequence similarity ≥94 %. Biochemical, serological and molecular biological properties demonstrate that this organism represents a novel species of the genus Mycoplasma , for which the name Mycoplasma hafezii sp. nov. is proposed; the type strain is M26 (NCTC 13928, DSM 27652).

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2019-02-01
2024-03-29
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References

  1. Bradbury JM. Poultry mycoplasmas: sophisticated pathogens in simple guise. Br Poult Sci 2005; 46:125–136 [View Article]
    [Google Scholar]
  2. Volokhov DV, Simonyan V, Davidson MK, Chizhikov VE. RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae. Mol Phylogenet Evol 2012; 62:515–528 [View Article][PubMed]
    [Google Scholar]
  3. Brown DR, May M, Bradbury JM, Johansson K-E. Class I. Mollicutes. In Krieg NR, Staley JT, Brown DR. (editors) Bergey's Manual of Systematic Bacteriology New York: Springer; 2011 pp. 568–644
    [Google Scholar]
  4. Lierz M, Hafez HM. Mycoplasma species in psittacine birds with respiratory disease. Vet Rec 2009; 164:629–630 [View Article][PubMed]
    [Google Scholar]
  5. Shimizu T, Erno H, Nagamoto H. Isolation and characterization of Mycoplasma columbinum and Mycoplasma columborale, two new species from pigeons. Int J Syst Evol Microbiol 1978; 28:538–546
    [Google Scholar]
  6. Möller Palau-Ribes F, Enderlein D, Hagen N, Herbst W, Hafez HM et al. Description and prevalence of Mycoplasma ciconiae sp. nov. isolated from white stork nestlings (Ciconia ciconia). Int J Syst Evol Microbiol 2016; 66:3477–3484 [View Article][PubMed]
    [Google Scholar]
  7. Assunção P, de Ponte Machado M, de La Fe C, Ramírez AS, Rosales RS et al. Prevalence of Pathogens in Great White Pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa. J Appl Anim Res 2007; 32:29–32 [View Article]
    [Google Scholar]
  8. Stenkat J, Schmidt V, Spergser J, Eilers A, Krautwald-Junghanns M-E et al. (editor) Untersuchungen zur aeroben bakteriellen Rachen- und Kloakenflora freilebender Wildvögel. 2 DVG-Tagung über Vogel- und Reptilienkrankheiten Hannover: Verlag der DVG Service GmbH; 2011
    [Google Scholar]
  9. Lierz M, Hagen N, Hernadez-Divers SJ, Hafez HM. Occurrence of mycoplasmas in free-ranging birds of prey in Germany. J Wildl Dis 2008; 44:845–850 [View Article][PubMed]
    [Google Scholar]
  10. Lierz M, Hagen N, Lueschow D, Hafez HM. Species-specific polymerase chain reactions for the detection of Mycoplasma buteonis, Mycoplasma falconis, Mycoplasma gypis, and Mycoplasma corogypsi in captive birds of prey. Avian Dis 2008; 52:94–99 [View Article][PubMed]
    [Google Scholar]
  11. Lierz M, Hagen N, Lueschow D, Hafez HM. Use of polymerase chain reactions to detect Mycoplasma gallisepticum, Mycoplasma imitans, Mycoplasma iowae, Mycoplasma meleagridis and Mycoplasma synoviae in birds of prey. Avian Pathol 2008; 37:471–476 [View Article][PubMed]
    [Google Scholar]
  12. Brown DR, Whitcomb RF, Bradbury JM. Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes). Int J Syst Evol Microbiol 2007; 57:2703–2719 [View Article][PubMed]
    [Google Scholar]
  13. Barile MF. Gram staining technique. In Razin S, Tully JG. (editors) Methods in Mycoplasmology New York: Academic Press, Inc; 1983 pp. 39–41
    [Google Scholar]
  14. Tully JG. Cloning and filtration techniques for mycoplasmas. In Razin S, Tully JG. (editors) Methods in Mycoplasmology New York: Academic Press, Inc; 1983 pp. 173–177
    [Google Scholar]
  15. Poveda JB. Biochemical characteristics in mycoplasma identification. In Miles R, Nicholas RAJ. (editors) Methods of Molecular Biology Totowa, New Jersey: Humana Press; 1998 pp. 69–78
    [Google Scholar]
  16. Konai M, Clark EA, Camp M, Koeh AL, Whitcomb RF. Temperature Ranges, Growth Optima, and Growth Rates of Spiroplasma (Spiroplasmataceae, class Mollicutes) Species. Curr Microbiol 1996; 32:314–319 [View Article][PubMed]
    [Google Scholar]
  17. Kotani H, McGarrity GJ. Rapid and simple identification of mycoplasmas by immunobinding. J Immunol Methods 1985; 85:257–267 [View Article][PubMed]
    [Google Scholar]
  18. Yavari CA. Studies on a Mycoplasma gallisepticum-like organism isolated from the Humboldt penguin (Spheniscus humboldti). University of Liverpool 2010
    [Google Scholar]
  19. Lierz M, Hagen N, Harcourt-Brown N, Hernandez-Divers SJ, Lüschow D et al. Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction. Avian Pathol 2007; 36:145–150 [View Article][PubMed]
    [Google Scholar]
  20. van Kuppeveld FJ, van der Logt JT, Angulo AF, van Zoest MJ, Quint WG et al. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 1992; 58:2606–2615[PubMed]
    [Google Scholar]
  21. Ramírez AS, Naylor CJ, Pitcher DG, Bradbury JM. High inter-species and low intra-species variation in 16S-23S rDNA spacer sequences of pathogenic avian mycoplasmas offers potential use as a diagnostic tool. Vet Microbiol 2008; 128:279–287 [View Article][PubMed]
    [Google Scholar]
  22. Stackebrandt E, Goebel BM. Taxonomic Note: a Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  23. Ziegler L, Palau-Ribes FM, Schmidt L, Lierz M. Occurrence and relevance of mycoplasma sturni in free-ranging corvids in germany. J Wildl Dis 2017; 53:228–234 [View Article][PubMed]
    [Google Scholar]
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