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Abstract

A novel Gram-stain-negative, strictly aerobic, rod-shaped motile bacterium with a single flagellum, designated strain WRAS1, was isolated from deep seawater of the Okinawa Trough. Growth occurred in the presence of 0.0–9.0 % NaCl (w/v; optimum, 3.0–4.0 %), at 4–45 °C (optimum, 28–37 °C) and pH 7.0–10.0 (optimum, pH 7.0–8.0). The major fatty acid (>10 % of total fatty acids) was summed feature 8, comprising C 6 and/or C 7. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids. The major respiratory quinone was ubiquinone-10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WRAS1 was in the genus and showed the highest 16S rRNA gene sequence similarity of 97.5 % to JCM13833. Genome relatedness between strain WRAS1 and JCM13833 was computed using both average nucleotide identity and DNA–DNA hybridization with values of 74.11 % and 22.70±2.3 %, respectively. The genomic DNA G+C content calculated from the genome sequence of strain WRAS1 was 65.6 %. On the basis of polyphasic analyses, strain WRAS1 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is WRAS1 (=CGMCC 1.16123=MCCC 1K03253 =KCTC 52696).

Funding
This study was supported by the:
  • National Key R and D Program of China (Award 2018YFC0310701)
  • National Natural Science Foundation of China (Award U1706208)
  • Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (Award 2016ASKJ14)
  • National Natural Science Foundation of China (Award 91751202)
  • Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (Award 2018SDKJ0406-4)
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2019-01-10
2021-08-01
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