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Abstract

A novel marine actinomycete, designated LHW63014, isolated from a marine sponge, Craniella species, collected in the South China Sea, was examined using a polyphasic approach. The 16S rRNA gene sequence of strain LHW63014 showed highest similarities to Jishengella endophytica 202201 (98.9 %), Micromonospora olivasterospora DSM 43868 (98.7 %), Micromonospora maris AB-18-032 (98.6 %) and Micromonospora gifhornensis DSM 44337 (98.6 %). Phylogenetic analyses of the family Micromonosporaceae based on the 16S rRNA and gyrB gene sequences indicated that strain LHW63014 and J. endophytica 202201 located within the genus Micromonospora . Morphological and chemotaxonomic characteristics confirmed their affiliation to the genus Micromonospora . Based on phenotypic characteristics, phylogenetic data and digital DNA–DNA hybridization results, strain LHW63014 could be distinguished from its closest taxa, representing a novel species of the genus Micromonospora , for which the name Micromonospora craniellae sp. nov., is proposed, with the type strain LHW63014 (=DSM 106193=CCTCC AA 2018012). It is also proposed that J. endophytica be transferred to genus Micromonospora as Micromonospora endophytica comb. nov. (type strain 202201 =CGMCC 4.5597=DSM 45430).

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2019-01-03
2020-01-21
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