1887

Abstract

Two strains of a psychrophilic basidiomycetous yeast species belonging to the genus Vishniacozyma were isolated from sediments and soil at the front of a retreating glacier on northern Ellesmere Island in the Canadian Arctic. Analysis of the large subunit D1/D2 region and the internal transcribed spacer (ITS) regions of the rDNA indicated that these strains represented a novel species. The closest relatives of the novel species were Vishniacozyma globispora and V. dimennae, which exhibited sequence similarities of 82.2 and 81.6 %, respectively, to the ITS region of the novel species, and contained 61 and 57 nt substitutions, respectively, in the D1/D2 domain, in comparison with the novel species. Strains of the novel species were able to grow at sub-zero temperatures and in vitamin-free medium. These characteristics allow the strains to survive and grow in perennially cold, oligotrophic habitats in the Canadian High Arctic. The name Vishniacozyma ellesmerensis sp. nov. is proposed. The type strain is JCM 32573 (=UAMH 11973=G3-4-8) and the MycoBank number is MB825501.

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2019-01-03
2024-03-28
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References

  1. Feller G, Gerday C. Psychrophilic enzymes: hot topics in cold adaptation. Nat Rev Microbiol 2003; 1:200–208 [View Article][PubMed]
    [Google Scholar]
  2. Vincent WF. Microbial Ecosystems of Antarctica Cambridge; Univers; ity; Press; 2004
    [Google Scholar]
  3. Welander U. Microbial degradation of organic pollutants in soil in a cold climate. Soil and Sediment Contamination: An International Journal 2005; 14:281–291 [View Article]
    [Google Scholar]
  4. Margesin R, Neuner G, Storey KB. Microbes C-Loving plants, and animals—fundamental and applied aspects. Naturwissenschaften 2007; 94:77–99
    [Google Scholar]
  5. di Menna ME. Yeasts in Antarctic soils. Antonie van Leeuwenhoek 1966; 32:29–38 [View Article][PubMed]
    [Google Scholar]
  6. Singh P, Tsuji M, Singh SM, Roy U, Hoshino T. Taxonomic characterization, adaptation strategies and biotechnological potential of cryophilic yeasts from ice cores of Midre Lovénbreen glacier, Svalbard, Arctic. Cryobiology 2013; 66:167–175 [View Article][PubMed]
    [Google Scholar]
  7. Tsuji M, Fujiu S, Xiao N, Hanada Y, Kudoh S et al. Cold adaptation of fungi obtained from soil and lake sediment in the Skarvsnes ice-free area, Antarctica. FEMS Microbiol Lett 2013; 346:121–130 [View Article][PubMed]
    [Google Scholar]
  8. Tsuji M, Yokota Y, Shimohara K, Kudoh S, Hoshino T. An application of wastewater treatment in a cold environment and stable lipase production of Antarctic basidiomycetous yeast Mrakia blollopis. PLoS One 2013; 8:e59376 [View Article][PubMed]
    [Google Scholar]
  9. Tsuji M, Uetake J, Tanabe Y. Changes in the fungal community of Austre Brøggerbreen deglaciation area, Ny-Ålesund, Svalbard, High Arctic. Mycoscience 2016; 57:448–451 [View Article]
    [Google Scholar]
  10. Buzzini P, Turk M, Perini L, Turchetti B, Gunde-Cimerman N et al. Yeasts in polar and subpolar habitats. In Buzzini P, Marc-André L, Yurkov A. (editors) Yeasts in Natural Ecosystems: Diversity Cham: Springer Nature; 2017
    [Google Scholar]
  11. Boekhout T, Sampaio JP, Bandoni RJ, Fell JW. Discussion of teleomorphic and anamorphic basidiomycetous yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeast a Taxonomic Study, 5th ed.. Elsevier; 2011 pp. 1339–1372
    [Google Scholar]
  12. Boekhout T, Fell JW, Vuillemin C. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeast a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 1661–1737
    [Google Scholar]
  13. Sampaio JP, Harrison R. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeast a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 1873–1927
    [Google Scholar]
  14. Buzzini P, Branda E, Goretti M, Turchetti B. Psychrophilic yeasts from worldwide glacial habitats: diversity, adaptation strategies and biotechnological potential. FEMS Microbiol Ecol 2012; 82:217–241 [View Article][PubMed]
    [Google Scholar]
  15. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  16. Tsuji M, Kudoh S, Hoshino T. Ethanol productivity of cryophilic basidiomycetous yeast Mrakia spp. correlates with ethanol tolerance. Mycoscience 2016; 57:42–50 [View Article]
    [Google Scholar]
  17. Vincent WF, Fortier D, Lévesque E, Boulanger-Lapointe N, Tremblay B et al. Extreme ecosystems and geosystems in the Canadian High Arctic: Ward Hunt Island and vicinity. Écoscience 2011; 18:236–261 [View Article]
    [Google Scholar]
  18. Katoh K, Rozewicki J, Yamada KD. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 2017bbx108 [View Article][PubMed]
    [Google Scholar]
  19. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeast a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 87–111
    [Google Scholar]
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