RT Journal Article SR Electronic(1) A1 Jin, Liying A1 Zhao, Yang A1 Song, Wei A1 Duan, Liping A1 Jiang, Shanwen A1 Wang, Xiangjing A1 Zhao, Junwei A1 Xiang, WenshengYR 2019 T1 Streptomyces inhibens sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 3 SP 688 OP 695 DO https://doi.org/10.1099/ijsem.0.003204 PB Microbiology Society, SN 1466-5034, AB A novel actinomycete, designated strain NEAU-D10T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Northeast Agricultural University in Harbin, Heilongjiang Province, north-east China. A polyphasic approach was employed to determine the status of strain NEAU-D10T. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces . The menaquinones detected were MK-9 (H6), MK-9 (H8) and MK-9 (H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and three unidentified lipids. The major fatty acids were identified as iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and iso-C14 : 0. Analysis of the 16S rRNA gene sequence showed that strain NEAU-D10T belongs to the genus Streptomyces with high sequence similarity to Streptomyces sioyaensis DSM 40032T (99.0 %) and Streptomyces auratus DSM 41897T (98.8 %). Moreover, multilocus sequence analysis based on five other housekeeping genes (atpD, gyrB, rpoB, recAand trpB) and the low level of DNA–DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strains, S. sioyaensis DSM 40032T and S. auratus DSM 41897T. It is concluded that the organism can be classified as representing a novel species of the genus Streptomyces , for which the name Streptomyces inhibens sp. nov. is proposed. The type strain is NEAU-D10T (=CGMCC 4.7469T=DSM 106197T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003204