A new species of the genus Trichococcus, strain Art1T, was isolated from a psychrotolerant syntrophic propionate-oxidizing consortium, obtained before from a low-temperature EGSB reactor fed with a mixture of VFAs (acetate, propionate and butyrate). The 16S rRNA gene sequence of strain Art1T was highly similar to those of other Trichococcus species (99.7–99.9 %) but digital DNA–DNA hybridization values were lower than those recommended for the delineation of a novel species, indicating that strain Art1T is a novel species of the genus Trichococcus. Cells of strain Art1T are non-motile cocci with a diameter of 0.5–2.0 µm and were observed singularly, in pairs, short chains and irregular conglomerates. Cells of Art1T stained Gram-positive and produced extracellular polymeric substances . Growth was optimal at pH 6–7.5 and cells could grow in a temperature range of from −2 to 30 °C (optimum 25–30 °C). Strain Art1T can degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate and ethanol. The genomic DNA G+C content of strain Art1T is 46.7 %. The major components of the cellular fatty acids are C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c. Based on genomic and physiological characteristics of strain Art1T, a new species of the genus Trichococcus, Trichococcusshcherbakoviae, is proposed. The type strain of Trichococcusshcherbakoviae is Art1T (=DSM 107162T = VKM B–3260T).
SchinkB. Fermentation of tartrate enantiomers by anaerobic bacteria, and description of two new species of strict anaerobes, Ruminococcus pasteurii and Ilyobacter tartaricus. Arch Microbiol1984; 139:409–414 [View Article]
JanssenPH, EversS, RaineyFA, WeissN, LudwigW et al.Lactosphaera gen. nov., a new genus of lactic acid bacteria, and transfer of Ruminococcus pasteurii Schink 1984 to Lactosphaera pasteurii comb. nov. Int J Syst Bacteriol1995; 45:565–571 [View Article][PubMed]
LiuJR, TannerRS, SchumannP, WeissN, McKenzieCA et al. Emended description of the genus Trichococcus, description of Trichococcus collinsii sp. nov., and reclassification of Lactosphaera pasteurii as Trichococcus pasteurii comb. nov. and of Ruminococcus palustris as Trichococcus palustris comb. nov. in the low-G+C Gram-positive bacteria. Int J Syst Evol Microbiol2002; 52:1113–1126 [View Article][PubMed]
PikutaEV, HooverRB, BejAK, MarsicD, WhitmanWB et al.Trichococcus patagoniensis sp. nov., a facultative anaerobe that grows at -5°C, isolated from penguin guano in Chilean Patagonia. Int J Syst Evol Microbiol2006; 56:2055–2062 [View Article][PubMed]
StrepisN, Sánchez-AndreaI, van GelderAH, van KruistumH, ShapiroN et al. Description of Trichococcus ilyis sp. nov. by combined physiological and in silico genome hybridization analyses. Int J Syst Evol Microbiol2016; 66:3957–3963 [View Article][PubMed]
DaiYM, ZhangLL, LiY, LiYQ, DengXH et al. Characterization of Trichococcus paludicola sp. nov. and Trichococcus alkaliphilus sp. nov., isolated from a high-elevation wetland, by phenotypic and genomic analyses. Int J Syst Evol Microbiol2018; 68:99–105 [View Article][PubMed]
KonstantinidisKT, TiedjeJM. Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci USA2005; 102:2567–2572 [View Article][PubMed]
GorisJ, KonstantinidisKT, KlappenbachJA, CoenyeT, VandammeP et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol2007; 57:81–91 [View Article][PubMed]
RegueiroL, VeigaP, FigueroaM, LemaJM, CarballaM. Influence of transitional states on the microbial ecology of anaerobic digesters treating solid wastes. Appl Microbiol Biotechnol2014; 98:2015–2027 [View Article][PubMed]
BaekG, KimJ, ChoK, BaeH, LeeC. The biostimulation of anaerobic digestion with (semi)conductive ferric oxides: their potential for enhanced biomethanation. Appl Microbiol Biotechnol2015; 99:10355–10366 [View Article][PubMed]
ZengT, ZhangS, GaoX, WangG, LensPNL et al. Assessment of bacterial community composition of anaerobic granular sludge in response to short-term uranium exposure. Microb Ecol2018; 76:648–659 [View Article][PubMed]
LettingaG, RebacS, ParshinaS, NozhevnikovaA, van LierJB et al. High-rate anaerobic treatment of wastewater at low temperatures. Appl Environ Microbiol1999; 65:1696–1702[PubMed]
StamsAJ, van DijkJB, DijkemaC, PluggeCM. Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl Environ Microbiol1993; 59:1114–1119[PubMed]
YarzaP, RichterM, PepliesJ, EuzebyJ, AmannR et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol2008; 31:241–250 [View Article][PubMed]
RichterM, Rosselló-MóraR, GlöcknerFO, PepliesJ. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics2015; 5;32:929–931
ChunJ, OrenA, VentosaA, ChristensenH, ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2017461–466
KoehorstJJ, SaccentiE, SchaapPJ, Martins dos SantosVAP, Suarez-DiezM. Protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics. F1000 Research1987; 2016:5
DoetschR. Determinative methods of light microscopy. In GerhardtP, MurrayRDE, CostilowRN, NesterEW, WoodWA et al. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 21–33
DuboisM, GillesKA, HamiltonJK, RebersPA, SmithF. Colorimetric method for determination of sugars and related substances. Anal Chem1956; 28:350–356 [View Article]