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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 2

Aeromicrobium lacus sp. nov., a novel actinobacterium isolated from a drinking-water reservoir Free

Abstract

An actinobacterial strain, designated CPCC 204604, was isolated from a water sample collected from Sancha Lake, a drinking-water reservoir in Sichuan Province, south-west China. Cells were strictly aerobic, Gram-stain-positive, non-spore-forming and non-motile. Polyphasic taxonomic analysis indicated that galactose and ribose were detected as the diagnostic sugars in the whole-cell hydrolysates, and ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan. MK-9(H4) was the predominant menaquinone. The phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The major fatty acids were C16 : 0, C16 : 0 2-OH, C18 : 1ω9c, C18 : 0 and 10-methyl C18:0. The genomic DNA G+C content was 71.2 mol%. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 204604 formed a distinct clade with Aeromicrobium ponti DSM 19178 within the lineage of the genus Aeromicrobium , with the highest 16S rRNA gene sequence similarity of 98.3 % to its closest neighbour. On the basis of the genotypic and phenotypic data, we conclude that strain CPCC 204604 represents a novel species of the genus Aeromicrobium , for which the name Aeromicrobium lacus sp. nov. is proposed with strain CPCC 204604 (=NBRC 112936=DSM 105424) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria , Aeromicrobium , Aeromicrobium lacus and polyphasic taxonomy
© 2019 IUMS
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2018-12-17
2024-09-15
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References

  1. Miller ES, Woese CR, Brenner S. Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol 1991; 41:363–368 [View Article][PubMed]
     [Google Scholar]
  2. Yoon JH, Lee CH, Oh TK. Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium. Int J Syst Evol Microbiol 2005; 55:2171–2175 [View Article][PubMed]
     [Google Scholar]
  3. Ber P, Trappen SV, Vandamme P, Trček J. Aeromicrobium choanae sp. nov., an actinobacterium isolated from the choana of a garden warbler. Int J Syst Evol Microbiol 2017; 67:367–361 [View Article][PubMed]
     [Google Scholar]
  4. Lee DW, Lee SD. Aeromicrobium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2008; 58:987–991 [View Article][PubMed]
     [Google Scholar]
  5. Tang Y, Zhou G, Zhang L, Mao J, Luo X et al. Aeromicrobium flavum sp. nov., isolated from air. Int J Syst Evol Microbiol 2008; 58:1860–1863 [View Article][PubMed]
     [Google Scholar]
  6. Kim MK, Park MJ, Im WT, Yang DC. Aeromicrobium ginsengisoli sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 2008; 58:2025–2030 [View Article][PubMed]
     [Google Scholar]
  7. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
     [Google Scholar]
  8. Sun Y, Chen HH, Sun HM, Ai MJ, Su J et al. Naumannella huperziae sp. nov., an endophytic actinobacterium isolated from Huperzia serrata (Thunb). Int J Syst Evol Microbiol 2017; 67:1867–1872 [View Article][PubMed]
     [Google Scholar]
  9. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  10. Magee CM, Rodeheaver G, Edgerton MT, Edlich RF. A more reliable gram staining technic for diagnosis of surgical infections. Am J Surg 1975; 130:341–346 [View Article][PubMed]
     [Google Scholar]
  11. Yuan LJ, Zhang YQ, Guan Y, Wei YZ, Li QP et al. Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 2008; 58:1180–1185 [View Article][PubMed]
     [Google Scholar]
  12. Lechevalier H, Lechevalier MP. Classification des actinomycetes aerobes basée sur leur morphologie et leur composition chimique. Ann Inst Pasteur (Paris) 1965; 108:662–673
     [Google Scholar]
  13. Lechevalier MP, Lechevalier HA. The chemotaxonomy of actinomycetes. In Dietz A, Thayer DW. (editors) Actinomycete Taxonomy (SIM Special Publication no. 6) Fairfax, VA: Society for Industrial Microbiology; 1980 pp. 227–291
     [Google Scholar]
  14. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477[PubMed]
     [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  16. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  17. Kroppenstedt RM. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20 Manhattan, NY: Academic Press; 1985 pp. 173–199
     [Google Scholar]
  18. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
     [Google Scholar]
  19. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  20. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0. molecular biology and evolution. Mol Biol Evol 2016; 33:1870–1874
     [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  22. Kluge AG, Farris JS. Quantitative phyletics and the evolution of anurans. Syst Zool 1969; 18:1–32 [View Article]
     [Google Scholar]
  23. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  25. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
  26. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  27. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464
     [Google Scholar]
  28. Kim SH, Yang HO, Sohn YC, Kwon HC. Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi. Int J Syst Evol Microbiol 2010; 60:2793–2798 [View Article][PubMed]
     [Google Scholar]
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Aeromicrobium lacus sp. nov., a novel actinobacterium isolated from a drinking-water reservoir
Int J Syst Evol Microbiol 69, 460 (2019); https://doi.org/10.1099/ijsem.0.003181
/content/journal/ijsem/10.1099/ijsem.0.003181
/content/journal/ijsem/10.1099/ijsem.0.003181
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