1887

Abstract

A Gram-stain-negative, rod-shaped, motile and strictly aerobic novel bacterial isolate, designated strain K1W11S-77, was obtained from a water sample that was collected from a karst cave in Guizhou province, PR China. The results of a phylogenetic analysis based on 16S rRNA gene sequences indicated that K1W11S-77 represented a member of the genus Crenobacter within the family Neisseriaceae of the phylum Proteobacteria . K1W11S-77 was phylogenetically closely related to Crenobacter luteus YIM 78141 (Their 16S rRNA gene sequence similarity is 95.02 %). Growth of K1W11S-77 occurred at 10–30 °C, at pH 7–9, and in the presence of 0–1 % (w/v) NaCl. The major cellular fatty acids were C12 : 0, C16 : 0, C18:1ω7c and summed feature 3. The major isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and one unidentified phospholipid. The genome of K1W11S-77 was 3.27 Mb long and encoded 3167 annotated genes. The DNA G+C content of the genomic DNA was 65.3 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, K1W11S-77 is considered to represent a novel species of the genus Crenobacter , for which the name Crenobacter cavernae sp. nov. is proposed. The type strain is K1W11S-77 (=CGMCC 1.13527=NBRC 113452).

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2018-12-17
2019-09-19
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