1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain C8-1, was isolated from the rhizosphere soil of Nicotiana tabacum L. collected from Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10–40 °C, at pH 6.0–8.0 and in the presence of up to 1 % (w/v) NaCl, with optimal growth at 30 °C and pH 7.0. The predominant isoprenoid quinone was Q-8. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1ω9c. The cellular polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, five unidentified phospholipids and two unidentified aminophospholipids. The genomic DNA G+C content was 70.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C8-1 should be assigned to the genus Lysobacter . 16S rRNA gene sequence similarity analysis showed that strain C8-1 was closely related to Lysobacter cavernae YIM C01544 (98.6 %), Lysobacter soli DCY21 (97.6 %), Lysobacter panacisoli CJ29 (97.3 %), Lysobacter firmicutimachus PB-6250 (97.3 %), Lysobacter niastensis GH41-7 (97.3 %) and Lysobacter gummosus KCTC 12132 (97.1 %). DNA–DNA hybridization data indicated that the isolate may represent a novel genomic species belonging to the genus Lysobacter . Polyphasic taxonomic characteristics indicated that strain C8-1 represents a novel species of the genus Lysobacter , for which the name Lysobacter tabacisoli sp. nov. is proposed. The type strain is C8-1 (=KCTC 62034=CGMCC 1.16271) .

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2018-12-12
2019-09-15
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