1887

Abstract

Two bacterial strains, YZYP 306 and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain YZYP 306 grew at 4–40 °C, while strain YZGP 509 grew at 4–42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0–12.0 and 0–15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6 (97.8 %) and Microbacterium sorbitolivorans SZDIS-1-1 (97.2 %). The average nucleotide identity value between YZYP 306 and M. indicum BBH6 was 78.3 %. The genomic DNA G+C contents of strains YZYP 306 and YZGP 509 were 68.49 and 68.53 mol%, respectively. The characteristic cell-wall amino acid was ornithine. Whole-cell sugars were galactose, mannose and ribose. The acyl type of the peptidoglycan was glycolyl. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major menaquinones were MK-10 and MK-11. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. These results are consistent with the classification of the two strains into the genus Microbacterium. On the basis of the evidence presented in this study, strains YZYP 306 and YZGP 509 are representatives of a novel species in the genus Microbacterium, for which the name Microbacterium suaedae sp. nov. is proposed. The type strain is YZYP 306 (=CGMCC 1.16261=KCTC 49101).

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2018-12-14
2019-12-09
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References

  1. Orla-Jensen S. The Lactic Acid Bacteria Copenhagen: Host & Sons; 1919
    [Google Scholar]
  2. Collins MD, Jones D, Kroppenstedt RM. Reclassification of Brevibacterium imperiale (steinhaus) and "Corynebacterium laevaniformans" (dias and bhat) in a redefined genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev.; comb. nov. Syst Appl Microbiol 1983;4:65–78 [CrossRef][PubMed]
    [Google Scholar]
  3. Takeuchi M, Hatano K. Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. In a redefined genus Microbacterium. Int J Syst Bacteriol 1998;48 Pt 3:739–747 [CrossRef][PubMed]
    [Google Scholar]
  4. Krishnamurthi S, Bhattacharya A, Schumann P, Dastager SG, Tang SK et al. Microbacterium immunditiarum sp. Nov., An actinobacterium isolated from landfill surface soil, and emended description of the genus Microbacterium. Int J Syst Evol Microbiol 2012;62:2187–2193 [CrossRef][PubMed]
    [Google Scholar]
  5. Alves A, Correia A, Igual JM, Trujillo ME. Microbacterium endophyticum sp. nov. and Microbacterium halimionae sp. nov., endophytes isolated from the salt-marsh plant Halimione portulacoides and emended description of the genus Microbacterium. Syst Appl Microbiol 2014;37:474–479 [CrossRef][PubMed]
    [Google Scholar]
  6. Fidalgo C, Riesco R, Henriques I, Trujillo ME, Alves A. Microbacterium diaminobutyricum sp. nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium. Int J Syst Evol Microbiol 2016;66:4492–4500 [CrossRef][PubMed]
    [Google Scholar]
  7. Kook M, Son HM, Yi TH. Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil. Int J Syst Evol Microbiol 2014;64:2267–2273 [CrossRef][PubMed]
    [Google Scholar]
  8. Lee JS, Lee KC, Park YH. Microbacterium koreense sp. nov., from sea water in the South Sea of Korea. Int J Syst Evol Microbiol 2006;56:423–427 [CrossRef][PubMed]
    [Google Scholar]
  9. Yan ZF, Lin P, Won KH, Yang JE, Li CT et al. Microbacterium hibisci sp. nov., isolated from rhizosphere of mugunghwa (Hibiscus syriacus L.). Int J Syst Evol Microbiol 2017;67:3564–3569 [CrossRef][PubMed]
    [Google Scholar]
  10. Suzuki K, Hamada M. Genus I. Microbacterium Orla-Jensen 1919,179AL emend. Takeuchi and Hatano 1998, 744 VP. In Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Suzuki K et al. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 5 New York: Springer; 2012; pp.814–852
    [Google Scholar]
  11. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  12. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  13. Gonzalez C, Gutierrez C, Ramirez C. Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 1978;24:710–715 [CrossRef][PubMed]
    [Google Scholar]
  14. Tang SK, Li WJ, Dong W, Zhang YG, Xu LH et al. Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica 2003;17:6–10 [CrossRef]
    [Google Scholar]
  15. Cai M, Wang L, Cai H, Li Y, Wang YN et al. Salinarimonas ramus sp. nov. and Tessaracoccus oleiagri sp. nov., isolated from a crude oil-contaminated saline soil. Int J Syst Evol Microbiol 2011;61:1767–1775 [CrossRef][PubMed]
    [Google Scholar]
  16. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  17. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007;23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  19. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  20. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  21. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  22. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  23. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  24. Luo R, Liu B, Xie Y, Li Z, Huang W et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 2012;1:18 [CrossRef][PubMed]
    [Google Scholar]
  25. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  26. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207
    [Google Scholar]
  27. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982;5:2359–2367 [CrossRef]
    [Google Scholar]
  28. Tang SK, Wang Y, Chen Y, Lou K, Cao LL et al. Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 2009;59:2025–2032 [CrossRef][PubMed]
    [Google Scholar]
  29. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29:319–322 [CrossRef]
    [Google Scholar]
  30. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970;20:435–443 [CrossRef]
    [Google Scholar]
  31. Uchida K, Kudo T, Suzuki KI, Nakase T. A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 1999;45:49–56 [CrossRef][PubMed]
    [Google Scholar]
  32. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  33. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980;48:459–470 [CrossRef]
    [Google Scholar]
  34. Tran PN, Tan NEH, Lee YP, Gan HM, Polter SJ et al. Whole-genome sequence and classification of 11 endophytic bacteria from poison ivy (Toxicodendron radicans). Genome Announc 2015;3:pii: e01319-15 [CrossRef]
    [Google Scholar]
  35. Shivaji S, Bhadra B, Rao RS, Chaturvedi P, Pindi PK et al. Microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the Chagos Trench, Indian Ocean. Int J Syst Evol Microbiol 2007;57:1819–1822 [CrossRef][PubMed]
    [Google Scholar]
  36. Meng YC, Liu HC, Yang LL, Kang YQ, Zhou YG et al. Microbacterium sorbitolivorans sp. nov., a novel member of Microbacteriaceae isolated from fermentation bed in pigpen. Int J Syst Evol Microbiol 2016;66:5556–5561 [CrossRef][PubMed]
    [Google Scholar]
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