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Abstract

A novel bacterial strain, designated WSJ-1, was isolated from geopark soil in Gansu province, PR China. The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01 (97.10 %), Nocardioides aquiterrae GW-9 (97.08 %) and Nocardioides terrigena DS-17 (96.94 %). Strain WSJ-1 grouped with N. terrigena DS-17 and N. sediminis MSL-01 in 16S rRNA gene-based phylogenetic trees. The DNA–DNA relatedness of WSJ-1/ N. sediminis JCM19559 and WSJ-1/ N. aquiterrae JCM11813 were 44.8 and 29.2 %, respectively. Average nucleotide identity values of whole genome sequences of WSJ-1/ N. terrigena KCTC19217 and WSJ-1/ N. sediminis KCTC19271 were 78.83 and 78.83 %, respectively. Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %. Strain WSJ-1 contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides , for which the name Nocardioides gansuensis sp. nov. is proposed. The type strain is WSJ-1 (=KCTC 49117=CCTCC AB 2018027).

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2018-12-14
2019-09-17
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