1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 1

Flavobacterium niveum sp. nov., isolated from a freshwater creek Free

Abstract

Strain TAPW14 was isolated from a freshwater creek in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TAPW14 belonged to the genus Flavobacterium and was most closely related to Flavobacterium akiainvivens IK-1 (96.6 % sequence identity) and Flavobacterium hauense BX12 (96.0 %) and less than 96 % sequence similarity to other members of the genus. Cells of strain TAPW14 were Gram-negative, strictly aerobic, motile by gliding, rod-shaped and formed white colonies. Optimal growth occurred at 20 °C, pH 7 and in the presence of 0.5 % NaCl. Strain TAPW14 contained summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0 and C16 : 0 as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three uncharacterized aminophospholipids, one uncharacterized phospholipid and one uncharacterized lipid. The major polyamine was homospermidine. The major isoprenoid quinone was MK-6. The DNA G+C content of the genomic DNA was 46.0 mol%. On the basis of the phylogenetic inference and phenotypic data, strain TAPW14 should be classified as a novel species, for which the name Flavobacteriumniveum sp. nov. is proposed. The type strain is TAPW14 (=BCRC 81055=LMG 30057=KCTC 52808).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidetes , Flavobacteriaceae , Flavobacteriales , Flavobacteriia , Flavobacterium niveum sp. nov. and freshwater creek
© 2019 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003150
2018-11-30
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/1/271.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003150&mimeType=html&fmt=ahah

References

  1. Ludwig W, Euzéby J, Whitman WB. Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. In Whitman W. (editor) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 4 Baltimore: Williams & Wilkins; 2011 pp. 21–24
     [Google Scholar]
  2. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. Genus II. Flavobacterium gen. nov. Bergey's Manual of Determinative Bacteriology, 1st ed. Baltimore: Williams & Wilkins; 1923 pp. 97–117
     [Google Scholar]
  3. Bernardet J-F, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom nov (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
     [Google Scholar]
  4. Dong K, Chen F, du Y, Wang G. Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense. Int J Syst Evol Microbiol 2013; 63:886–892 [View Article][PubMed]
     [Google Scholar]
  5. Kang JY, Chun J, Jahng KY. Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium. Int J Syst Evol Microbiol 2013; 63:1633–1638 [View Article][PubMed]
     [Google Scholar]
  6. Kuo I, Saw J, Kapan DD, Christensen S, Kaneshiro KY et al. Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium. Int J Syst Evol Microbiol 2013; 63:3280–3286 [View Article][PubMed]
     [Google Scholar]
  7. Bernardet JF, Bowman JP. The genus Flavobacterium. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E et al. (editors) The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd ed. vol. 7 New York, NY: Springer; 2006 pp. 481–531
     [Google Scholar]
  8. Bernardet JF, Bowman JP. Genus I. Flavobacterium Bergey et al. 1923. In Whitman W. (editor) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 4 Baltimore: Williams & Wilkins; 19232011 pp. 112–154
     [Google Scholar]
  9. Liu Y, Jin JH, Zhou YG, Liu HC, Liu ZP. Flavobacterium caeni sp. nov., isolated from a sequencing batch reactor for the treatment of malachite green effluents. Int J Syst Evol Microbiol 2010; 60:417–421 [View Article][PubMed]
     [Google Scholar]
  10. Chen WM, Laevens S, Lee TM, Coenye T, de Vos P et al. Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 2001; 51:1729–1735 [View Article][PubMed]
     [Google Scholar]
  11. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
     [Google Scholar]
  12. Anzai Y, Kudo Y, Oyaizu H. The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. Int J Syst Bacteriol 1997; 47:249–251 [View Article][PubMed]
     [Google Scholar]
  13. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  14. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
     [Google Scholar]
  15. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
     [Google Scholar]
  16. Sheu SY, Su CL, Kwon SW, Chen WM. Flavobacterium amniphilum sp. nov., isolated from a stream. Int J Syst Evol Microbiol 2017; 67:5179–5186 [View Article][PubMed]
     [Google Scholar]
  17. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  18. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparativesystematic. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR et al. (editors) Methods for General and Molecular Bacteriology, 3rd ed. Washington, DC: American Society for Microbiology; 2007 pp. 330–393
     [Google Scholar]
  19. Wen CM, Tseng CS, Cheng CY, Li YK. Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnol Appl Biochem 2002; 35:213–219 [View Article][PubMed]
     [Google Scholar]
  20. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  21. Nokhal T-H, Schlegel HG. Taxonomic study of Paracoccus denitrificans. Int J Syst Bacteriol 1983; 33:26–37 [View Article]
     [Google Scholar]
  22. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc 1990
     [Google Scholar]
  23. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  24. Collins MD. Isoprenoid quinones. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 265–309
     [Google Scholar]
  25. Busse J, Auling G. Polyamine pattern as a chemotaxonomic marker within the proteobacteria. Syst Appl Microbiol 1988; 11:1–8 [View Article]
     [Google Scholar]
  26. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 1997; 47:698–708 [View Article]
     [Google Scholar]
  27. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics Chichester: Wiley; 1994 pp. 121–161
     [Google Scholar]
  28. Dong K, Xu B, Zhu F, Wang G. Flavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum, Flavobacterium beibuense and Flavobacterium rivuli. Int J Syst Evol Microbiol 2013; 63:3237–3242 [View Article][PubMed]
     [Google Scholar]
  29. Fujii D, Nagai F, Watanabe Y, Shirasawa Y. Flavobacterium longum sp. nov. and Flavobacterium urocaniciphilum sp. nov., isolated from a wastewater treatment plant, and emended descriptions of Flavobacterium caeni and Flavobacterium terrigena. Int J Syst Evol Microbiol 2014; 64:1488–1494 [View Article][PubMed]
     [Google Scholar]
  30. Singh H, du J, Won K, Yang JE, Akter S et al. Flavobacterium vireti sp. nov., isolated from soil. Antonie van Leeuwenhoek 2015; 107:1421–1428 [View Article][PubMed]
     [Google Scholar]
  31. Sheu SY, Chen YL, Chen WM. Flavobacterium verecundum sp. nov., isolated from a freshwater river. Int J Syst Evol Microbiol 2016; 66:3337–3344 [View Article][PubMed]
     [Google Scholar]
  32. Chen WM, Su CL, Sheu SY. Flavobacterium dispersum sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 2017; 67:4416–4423 [View Article][PubMed]
     [Google Scholar]
  33. Cai H, Zeng Y, Wang Y, Cui H, Jiang H. Flavobacterium cyanobacteriorum sp. nov., isolated from cyanobacterial aggregates in a eutrophic lake. Int J Syst Evol Microbiol 2018; 68:1279–1284 [View Article][PubMed]
     [Google Scholar]
  34. Ali Z, Cousin S, Frühling A, Brambilla E, Schumann P et al. Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet. Int J Syst Evol Microbiol 2009; 59:2610–2617 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003150
Loading
Flavobacterium niveum sp. nov., isolated from a freshwater creek
Int J Syst Evol Microbiol 69, 271 (2019); https://doi.org/10.1099/ijsem.0.003150
/content/journal/ijsem/10.1099/ijsem.0.003150
/content/journal/ijsem/10.1099/ijsem.0.003150
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error