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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 69, Issue 1

Kistimonas alittae sp. nov., a gammaproteobacterium isolated from the marine annelid Alitta succinea Free

Abstract

A novel Gram-stain-negative, rod-shaped, motile, non-spore-forming, facultatively anaerobic marine bacterium was isolated from the gastrointestinal tract of the sandworm Alitta succinea collected from Grice Cove, South Carolina, USA. The strain was arginine dihydrolase-positive, and oxidase- and catalase-positive. Growth occurred between 10 and 37 °C, with optimal growth occurring between 30 and 32 °C. Comparative 16S rRNA gene sequence analysis showed its nearest neighbours are members of the genus Kistimonas of the family Hahellaceae , which is found in the order Oceanospirillales , class Gammaproteobacteria . The closest related species was Kistimonas asteriae KMD 001 with 16S rRNA gene sequence similarity of 99.0 %. However, DNA–DNA hybridization between these strains revealed less than 70 % DNA–DNA relatedness, supporting the novel species status of the strain. The major fatty acids were C16 : 0, C18 : 0, C18 : 1ω7c and a summed feature that contained C16 : 1ω6c/C16 : 1ω7c. The major respiratory quinone was ubiquinone-9 and the predominant polar lipids were phosphatidylserine, phosphoethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 52.5 mol%. Based on the data presented, strain BGP-2 is considered to represent a novel member of the genus Kistimonas , for which the name Kistimonas alittae sp. nov. is proposed. The type strain is BGP-2 (=CCUG 65711=JCM 30010).

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Keyword(s): Alitta succinea , biosurfactant , deposit feeder , Kistimonas , sandworm and surfactant-resistant
© 2019 IUMS
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2018-11-28
2025-03-20
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Kistimonas alittae sp. nov., a gammaproteobacterium isolated from the marine annelid Alitta succinea
Int J Syst Evol Microbiol 69, 235 (2019); https://doi.org/10.1099/ijsem.0.003137
/content/journal/ijsem/10.1099/ijsem.0.003137
/content/journal/ijsem/10.1099/ijsem.0.003137
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