1887

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Volume 69, Issue 1

Capsulimonas corticalis gen. nov., sp. nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord. nov., of the class Armatimonadia of the phylum Armatimonadetes Free

Abstract

An aerobic bacterial strain designated AX-7 was isolated from the trunk surface of a Japanese beech (Fagus crenata). Cells of strain AX-7 were Gram-stain-negative, non-spore-forming, non-motile rods (1.0–1.2 µm in width and 1.2–3.0 µm in length) with peritrichous fimbriae. Cells were capsulated, and a number of them were surrounded by a thick slime layer. During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer. The temperature range for growth was 10–37 °C, with an optimum at 30 °C. The pH range for growth was 5.0–7.0, with an optimum at pH 6.0. Strain AX-7 used various sugars, including polysaccharides, and yeast extract as growth substrates. Strain AX-7 contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids. Four unidentified phospholipids and 11 unidentified polar lipids constituted the polar lipids. The DNA G+C content was 61.0 mol%. The cell-wall peptidoglycan contained ll-diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7 belonged to the class Armatimonadia , its closest relative being Armatimonas rosea YO-36, with sequence similarity of 88.1%. Based on data from this polyphasic study, we propose that strain AX-7 represents a new genus of a novel species within the novel order Capsulimonadales ord. nov. of the class Armatimonadia , for which the name Capsulimonas corticalis gen. nov., sp. nov. is proposed. The type strain of C. corticalis is AX-7 (=DSM 105890=NBRC 113044).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Armatimonadetes , Armatimonadia and Fagus crenata
© 2019 IUMS
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2018-11-26
2023-09-24
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Capsulimonas corticalis gen. nov., sp. nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord. nov., of the class Armatimonadia of the phylum Armatimonadetes
Int J Syst Evol Microbiol 69, 220 (2019); https://doi.org/10.1099/ijsem.0.003135
/content/journal/ijsem/10.1099/ijsem.0.003135
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