Arenibacillus arenosus gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from sea sand Free

Abstract

A Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain, designated CAU 1304, was isolated from sea sand. Strain CAU 1304 grew optimally at pH 8.5 and 30 °C and in the presence of 1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1304 belonged to the family Rhodobacteraceae , and was most closely related to Roseicitreum antarcticum ZS2-28 (96.54 % similarity) and Rhodobacter veldkampii ATCC 35703 (96.46 %). The major fatty acid was C18 : 1ω7c and the respiratory quinone was Q-10. The polar lipids were composed of phosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid. The DNA G+C content was 55.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that strain CAU 1304 represents a novel species of a new genus of the family Rhodobacteraceae , for which the name Arenibacillus arenosus gen. nov., sp. nov. is proposed. The type strain of Arenibacillus arenosus is CAU 1304 (=KCTC 42827=NBRC 113022).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003121
2018-11-20
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/69/1/153.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003121&mimeType=html&fmt=ahah

References

  1. Garrity GM, Bell JA, Lilburn T. Order VIII. Oceanospirillales ord. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria) New York: Springer; 2005 pp. 270
    [Google Scholar]
  2. Simon M, Scheuner C, Meier-Kolthoff JP, Brinkhoff T, Wagner-Döbler I et al. Phylogenomics of Rhodobacteraceae reveals evolutionary adaptation to marine and non-marine habitats. Isme J 2017; 11:1483–1499 [View Article][PubMed]
    [Google Scholar]
  3. Rosenberg E, Delong EF, Lory S, Stackebrandt E, Thompson F et al. The Prokaryotes, Alphaproteobacteria and Betaproteobacteria, 4th ed. Berlin: Springer-Verlag; 2014
    [Google Scholar]
  4. Yu Y, Yan SL, Li HR, Zhang XH. Roseicitreum antarcticum gen. nov., sp. nov., an aerobic bacteriochlorophyll a-containing alphaproteobacterium isolated from Antarctic sandy intertidal sediment. Int J Syst Evol Microbiol 2011; 61:2173–2179 [View Article][PubMed]
    [Google Scholar]
  5. Hansen TA, Imhoff JF. Rhodobacter veldkampii, a new species of phototrophic purple nonsulfur bacteria. Int J Syst Bacteriol 1985; 35:115–116 [View Article]
    [Google Scholar]
  6. Gordon RE, Mihm JM. Identification of Nocardia caviae (Erikson) nov. comb.*. Ann N Y Acad Sci 1962; 98:628–636 [View Article]
    [Google Scholar]
  7. Lane D. 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics 1991
    [Google Scholar]
  8. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  9. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  10. Felsenstein J. PHYLIP-phylogeny inference package (Version 3.2). Cladistics 1989; 5:164–166
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  13. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  14. Labrenz M, Collins MD, Lawson PA, Tindall BJ, Schumann P et al. Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 1999; 49:137–147 [View Article][PubMed]
    [Google Scholar]
  15. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  16. Cappuccino J, Sherman N. Microbiology: a Laboratory Manual, 6th ed. San Francisco: Benjamin Cummings Pearson Education; 2002
    [Google Scholar]
  17. Wang B, Tan T, Shao Z. Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2009; 59:1116–1121 [View Article][PubMed]
    [Google Scholar]
  18. Lanyi B. Classical and rapid identification methods for medically important bacteria. Method Microbiol 1987; 19:1–67
    [Google Scholar]
  19. Smibert R. Phenotypic characterization. In Methods for general and molecular bacteriology 1994
    [Google Scholar]
  20. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980; 188:221–233 [View Article]
    [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  22. Komagata K, Suzuki K-I. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1988; 19:161–207
    [Google Scholar]
  23. Venkata Ramana V, Sasikala C, Ramana C. Rhodobacter maris sp. nov., a phototrophic alphaproteobacterium isolated from a marine habitat of India. Int J Syst Evol Microbiol 2008; 58:1719–1722 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003121
Loading
/content/journal/ijsem/10.1099/ijsem.0.003121
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed