1887

Abstract

Three strains of a novel basidiomycetous yeast were isolated from the Xishuangbanna Tropical Rainforest, Yunnan Province, PR China. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions indicated that the novel species represents a member of the genus Vanrija. It differed from the most closely related known species, Vanrija albida CBS 2839, by 1.5 % sequence divergence (seven substitutions and two gaps out of 597 bp) in the D1/D2 domains and by 7.4 % sequence divergence (17 substitutions and 20 gaps over 495 bp) in the ITS regions, respectively. The three strains of the novel species reproduced asexually, and no mating could be found. In contrast to V. albida, the novel yeast species was able to assimilate d-glucosamine, inulin, erythritol and galactitol and unable to assimilate raffinose. The name Vanrija jinghongensis sp. nov. is proposed to accommodate these strains, with NYNU 17910 (=CICC 33269=CBS 15229) as the type strain.

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2018-11-14
2019-10-21
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References

  1. Moore RT. Taxonomic proposals for the classification of marine yeasts and other yeast-like fungi including the smuts. Botanica Marina 1980;23:361–373
    [Google Scholar]
  2. Weiss M, Bauer R, Sampaio JP, Oberwinkler F. Tremellomycetes and related groups. In McLaughlin DJ, Spatafora JW. (editors) Systematics and Evolution, The Mycota VII Part A Berlin: Springer-Verlag Press; 2014; pp.349–350
    [Google Scholar]
  3. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015;81:85–147 [CrossRef][PubMed]
    [Google Scholar]
  4. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015;81:1–26 [CrossRef][PubMed]
    [Google Scholar]
  5. Zheng J, Liu KF, Liu XJ, Zhang L, Hui FL. Deakozyma yunnanensis sp. nov., a novel yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2017;67:2436–2439 [CrossRef][PubMed]
    [Google Scholar]
  6. Ren YC, Liu ST, Li Y, Hui FL. Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood. Int J Syst Evol Microbiol 2015;65:2875–2881 [CrossRef][PubMed]
    [Google Scholar]
  7. Wang Y, Ren YC, Li Y, Hui FL. Molecular phylogeny and taxonomy of Yamadazyma dushanensis f.a., sp. nov., a cellobiose-fermenting yeast species from China. Curr Microbiol 2015;71:268–273 [CrossRef][PubMed]
    [Google Scholar]
  8. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – a Taxonomic Study, 5th ed.vol. 1 Amsterdam: Elsevier; 2011; pp.87–110
    [Google Scholar]
  9. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  10. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: a Guide to Methods and Applications New York: Academic Press; 1990; pp.315–322
    [Google Scholar]
  11. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  12. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  13. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;10:512–526 [CrossRef][PubMed]
    [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  16. Fonseca A, Boekhout T, Fell JW. Cryptococcus Vuillemin (1901). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – a Taxonomic Study, 5th ed.vol. 2 Amsterdam: Elsevier; 2011; pp.1661–1737
    [Google Scholar]
  17. Liu YR, Huang LY, Young SS, Chang CF, Lee CF. Asterotremella meifongana sp. nov. and Asterotremella nantouana sp. nov., two anamorphic basidiomycetous yeasts isolated from soil and mushrooms. Antonie van Leeuwenhoek 2011;99:643–650 [CrossRef][PubMed]
    [Google Scholar]
  18. Vogelmann SA, Chaves S, Hertel C. Cryptococcus thermophilus sp. nov., isolated from cassava sourdough. Int J Syst Evol Microbiol 2012;62:1715–1720 [CrossRef][PubMed]
    [Google Scholar]
  19. Prillinger H, Lopandic K, Sugita T, Wuczkowski M. Asterotremella gen. nov. albida, an anamorphic tremelloid yeast isolated from the agarics Asterophora lycoperdoides and Asterophora parasitica. J Gen Appl Microbiol 2007;53:167–175 [CrossRef][PubMed]
    [Google Scholar]
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