1887

Abstract

Two novel, obligately anaerobic, Gram-stain-negative, rod or coccoid-shaped bacteria, designated strains 4NBBH2 and 6FBBBH3, were isolated from faecal samples of a healthy Japanese woman and man. The 16S rRNA gene sequence analysis showed that these strains represent a distinct lineage within the family Sutterellaceae . Strain 4NBBH2 formed a monophyletic branch between the genera Parasutterella and Sutterella , with sequence similarity to Sutterella stercoricanis CCUG 47620 (92.6 %), followed by Sutterella wadsworthensis WAL 7877 (92.4 %), Sutterella parvirubra YIT 11816 (92.1 %) and Parasutterella secunda YIT 12071 (91.8 %). Strain 6FBBBH3 was affiliated to the genus Sutterella , with highest similarity to S. stercoricanis CCUG 47620 (97.1 %), followed by S. parvirubra YIT 11816 (96.6 %) and S. wadsworthensis WAL 7877 (95.2 %). Strains 4NBBH2 and 6FBBBH3 were asaccharolytic. Analysis of fatty acids revealed that strain 4NBBH2 could be differentiated from Sutterella species (including strain 6FBBBH3) by the presence of a low concentration of C16 : 1ω7c. The major respiratory quinones of strain 4NBBH2 were menaquinone (MK)-6 and methylmenaquinone (MMK)-6, whereas those of strain 6FBBBH3 were MK-5 and MMK-5. The G+C content of the genomic DNA of strains 4NBBH2 and 6FBBBH3 were 56.9 and 62.8 mol%, respectively. On the basis of the collected data, strain 4NBBH2 represents a novel species in a novel genus of the family Sutterellaceae , for which the name Mesosutterella multiformis gen. nov., sp. nov. is proposed. The type strain is 4NBBH2 (=JCM 32464=DSM 106860). We also propose a novel Sutterella species, Sutterella megalosphaeroides sp. nov., for strain 6FBBBH3 (=JCM 32470=DSM 106861).

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2018-11-05
2019-12-08
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