RT Journal Article SR Electronic(1) A1 Li, Xin A1 Salam, Nimaichand A1 Li, Jia-Ling A1 Chen, Yi-Min A1 Yang, Zi-Wen A1 Han, Ming-Xian A1 Mou, Xiaozhen A1 Xiao, Min A1 Li, Wen-JunYR 2019 T1 Aestuariivirga litoralis gen. nov., sp. nov., a proteobacterium isolated from a water sample, and proposal of Aestuariivirgaceae fam. nov. JF International Journal of Systematic and Evolutionary Microbiology, VO 69 IS 2 SP 299 OP 306 DO https://doi.org/10.1099/ijsem.0.003087 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, non-motile, short rod and aerobic bacterium, designated strain SYSU M10001T, was isolated from a water sample collected from the coastal region of Pearl River Estuary, Guangdong Province, PR China. Strain SYSU M10001T showed optimal growth at 28 °C, pH 7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences and concatenation of 20 protein markers revealed a distinct lineage for strain SYSU M10001T in the order Rhizobiales . Strain SYSU M10001T showed highest 16S rRNA gene sequence similarities to Hyphomicrobium nitrativorans NL23T (91.1 %) and Hyphomicrobium hollandicum IFAM KB-677T (91.1 %). The respiratory ubiquinone was Q-8. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and three unidentified lipids. The predominant cellular fatty acids identified were C19 : 0cyclo ω8c, summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0. The G+C content was determined to be 65.5 % (genome). On the basis of differences in the phenotypic, physiological and biochemical characteristics, and results of the phylogenetic analyses, strain SYSU M10001T is proposed to represent a novel species in a novel genus for which the name Aestuariivirga litoralis gen. nov., sp. nov. The type strain of the type species Aestuariivirga litoralis is SYSU M10001T (=NBRC 112960T=KCTC 52945T). Besides, the distinct phylogenetic lineage and the distinct chemotaxonomic profile among the families in the order Rhizobiales indicated that strain SYSU M10001T should represent a new family for which the name Aestuariivirgaceae fam. nov. is proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003087