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Abstract

A Gram-stain-negative Rhodobacterales strain, designated as FSX-11, was isolated from surface seawater of Xiamen port in China. Strain FSX-11 showed less than 96.5 % 16S rRNA gene sequence similarity to the type strains of species with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel isolate formed a distinct monophyletic clade within the family Rhodobacteriaceae and clustered distantly with the genera Thalassobius and Marivita . Cells of strain FSX-11 were non-motile, oval-shaped and facultative anaerobic. Optimal growth occurred at 20–30 °C, at pH 7.0–8.0 and in the presence of 2–3 % NaCl (w/v). The major respiratory quinone was ubiquinone-10. Summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), 11-methyl C18 : 1ω7c and C16 : 0 were the major fatty acids. The DNA G+C content of strain FSX-11 was 58.7 mol%. On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics and 16S rRNA gene signature nucleotide patterns, strain FSX-11 represents a novel species in a novel genus within the family Rhodobacteraceae , for which the name Litorivita pollutaquae gen. nov., sp. nov. is proposed. The type strain is FSX-11 (=JCM 32715=MCCC 1K03503).

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2018-10-19
2020-01-22
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References

  1. Garrity A. List of new names and new combinations previously effectively, but not validly, published. Gen 2006;1:28
    [Google Scholar]
  2. Buchan A, González JM, Moran MA. Overview of the marine Roseobacter lineage. Appl Environ Microbiol 2005;71:5665–5677 [CrossRef][PubMed]
    [Google Scholar]
  3. Rappé MS, Vergin K, Giovannoni SJ. Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. FEMS Microbiol Ecol 2000;33:219–232 [CrossRef][PubMed]
    [Google Scholar]
  4. Yang Y, Sun J, Tang K, Lin D, Li C et al. Ponticoccus lacteus sp. nov. of the family Rhodobacteraceae, isolated from surface seawater. Int J Syst Evol Microbiol 2015;65:1247–1250 [CrossRef][PubMed]
    [Google Scholar]
  5. Park S, Park JM, Kang CH, Kim SG, Yoon JH. Pseudoseohaeicola caenipelagi gen. nov., sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2015;65:1819–1824 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhang DC, Neuner K, Wu J, Yao J, Margesin R. Puniceibacterium sediminis sp. nov., from intertidal sediment. Int J Syst Evol Microbiol 2015;65:1462–1466 [CrossRef][PubMed]
    [Google Scholar]
  7. Li G, Lai Q, du Y, Liu X, Sun F et al. Aestuariivita atlantica sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2015;65:3281–3285 [CrossRef][PubMed]
    [Google Scholar]
  8. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae. In The Prokaryotes Springer; 2014; pp.439–512
    [Google Scholar]
  9. Zhang G, Wang S, Wang L. Sediminivirga luteola gen. nov., sp. nov., a member of the family Brevibacteriaceae, isolated from marine sediment. Int J Syst Evol Microbiol 2016;66:1494–1498 [CrossRef][PubMed]
    [Google Scholar]
  10. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  12. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  13. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  15. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992;9:945–967
    [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  17. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics 2008;24:713–714 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  19. Hwang CY, Bae GD, Yih W, Cho BC. Marivita cryptomonadis gen. nov., sp. nov. and Marivita litorea sp. nov., of the family Rhodobacteraceae, isolated from marine habitats. Int J Syst Evol Microbiol 2009;59:1568–1575 [CrossRef][PubMed]
    [Google Scholar]
  20. Arahal DR, Macián MC, Garay E, Pujalte MJ. Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 2005;55:2371–2376 [CrossRef][PubMed]
    [Google Scholar]
  21. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  22. Liao H, Li Y, Zhang M, Lin X, Lai Q et al. Altererythrobacter mangrovi sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2017;67:4851–4856 [CrossRef][PubMed]
    [Google Scholar]
  23. Kates M. Radioisotopic techniques in lipidology. Techniques of lipidology 2 rev ed 1986
    [Google Scholar]
  24. Collins MD. Isoprenoid quinone analysis in bacterial classification and identification. In Minnikin DE, Goodfellow M. (editors) Chemical Methods in Bacterial Systematics London Academic Press; 1985; pp.267–285
    [Google Scholar]
  25. Wu C. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology 1989;16:176–178
    [Google Scholar]
  26. Sasser M. Identification of bacteria by gas chrosmatography of cellular fatty acids. 1990
  27. Park S, Lee MH, Lee JS, Oh TK, Yoon JH. Thalassobius maritimus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012;62:8–12 [CrossRef][PubMed]
    [Google Scholar]
  28. Rüger HJ, Höfle MG. Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 1992;42:133–143 [CrossRef][PubMed]
    [Google Scholar]
  29. Uchino Y, Hirata A, Yokota A, Sugiyama J. Reclassification of marine Agrobacterium species: Proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 1998;44:201–210 [CrossRef][PubMed]
    [Google Scholar]
  30. Yi H, Chun J. Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 2006;44:171–176[PubMed]
    [Google Scholar]
  31. Park S, Jung YT, Won SM, Park JM, Yoon JH. Thalassobius aquaeponti sp. nov., an alphaproteobacterium isolated from seawater. Antonie van Leeuwenhoek 2014;106:535–542 [CrossRef][PubMed]
    [Google Scholar]
  32. Nogi Y, Mori K, Makita H, Hatada Y. Thalassobius abyssi sp. nov., a marine bacterium isolated from cold-seep sediment. Int J Syst Evol Microbiol 2016;66:574–579 [CrossRef][PubMed]
    [Google Scholar]
  33. Park I, Cha IT, Seo MJ. Thalassobius litorarius sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 2016;66:1666–1672 [CrossRef][PubMed]
    [Google Scholar]
  34. Yoon JH, Kang SJ, Lee SY, Jung YT, Lee JS et al. Marivita hallyeonensis sp. nov., isolated from seawater, reclassification of Gaetbulicola byunsanensis as Marivita byunsanensis comb. nov. and emended description of the genus Marivita Hwang et al. 2009. Int J Syst Evol Microbiol 2012;62:839–843 [CrossRef][PubMed]
    [Google Scholar]
  35. Yoon JH, Kang SJ, Jung YT, Oh TK. Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 2010;60:196–199 [CrossRef][PubMed]
    [Google Scholar]
  36. Budinoff CR, Dunlap JR, Hadden M, Buchan A. Marivita roseacus sp. nov., of the family Rhodobacteraceae, isolated from a temperate estuary and an emended description of the genus Marivita. J Gen Appl Microbiol 2011;57:259–267 [CrossRef][PubMed]
    [Google Scholar]
  37. Yoon JH, Kang SJ, Lee JS. Marivita geojedonensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2013;63:423–427 [CrossRef][PubMed]
    [Google Scholar]
  38. Zhong ZP, Liu Y, Hou TT, Liu HC, Zhou YG et al. Marivita lacus sp. nov., isolated from a saline lake. Int J Syst Evol Microbiol 2015;65:1889–1894 [CrossRef][PubMed]
    [Google Scholar]
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