%0 Journal Article %A Wang, Xiaoxia %A Yang, Jing %A Lu, Shan %A Lai, Xin-He %A Jin, Dong %A Pu, Ji %A Zhang, Gui %A Huang, Ying %A Zhu, Wentao %A Wu, Xiaomin %A Liang, Hao %A Xu, Jianguo %T Nocardioides houyundeii sp. nov., isolated from Tibetan antelope faeces %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 12 %P 3874-3880 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003076 %K Tibetan antelope %K wild life %K Qinghai-Tibet Plateau %K Nocardioides houyundeii %I Microbiology Society, %X In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78T and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai–Tibet Plateau, China. The cells were aerobic, oxidase-negative and catalase-positive. When cultured on brain–heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex. Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78T and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492T (98.3 %), Nocardioides gilvus XZ17T (97.4 %) and Nocardioides daejeonensis JCM 16922T (97.4 %). The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95–96 % threshold. The DNA G+C content of strains 78T and 601 were 71.2 and 71.3 mol% respectively. MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan. Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c. These data supported the affiliation of strains 78T and 601 to genus Nocardioides. Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioides houyundeii sp. nov. The type strain is 78T (=CGMCC 4.7461T=DSM 106424T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003076