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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 12

Nocardioides houyundeii sp. nov., isolated from Tibetan antelope faeces Free

Abstract

In the present study, we describe two novel Gram-stain-positive, irregular rod-shaped bacterial strains, 78 and 601, that had been isolated from the faeces of Tibetan antelopes at the Hoh Xil Nature Reserve, Qinghai–Tibet Plateau, China. The cells were aerobic, oxidase-negative and catalase-positive. When cultured on brain–heart infusion agar supplemented with 5 % sheep blood, colonies were cream in colour, circular, smooth and convex. Phylogenetic analysis based on the full-length 16S rRNA sequences revealed that type strain 78 and strain 601 belong to the genus Nocardioides, sharing the highest similarity to Nocardioides solisilvae JCM 31492 (98.3 %), Nocardioides gilvus XZ17 (97.4 %) and Nocardioides daejeonensis JCM 16922 (97.4 %). The average nucleotide identity values between the two novel strains and the three closely related type strains of the genus Nocardioides were lower than the 95–96 % threshold. The DNA G+C content of strains 78 and 601 were 71.2 and 71.3 mol% respectively. MK-8 (H4) was the predominant respiratory quinone and ll-2,6-diaminopimelic acid was the diagnostic diamino acid in its cell-wall peptidoglycan. Its polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The main whole-cell sugars were rhamnose, xylose and galactose and the major fatty acids (>10 %) were C17 : 1ω8c, iso-C16 : 0 and C18 : 1ω9c. These data supported the affiliation of strains 78 and 601 to genus Nocardioides. Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, we propose a novel species named Nocardioides houyundeii sp. nov. The type strain is 78 (=CGMCC 4.7461=DSM 106424).

  • Received:
  • Accepted:
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Keyword(s): Nocardioides houyundeii , Qinghai-Tibet Plateau , Tibetan antelope and wild life
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2018-10-26
2024-04-19
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Nocardioides houyundeii sp. nov., isolated from Tibetan antelope faeces
Int J Syst Evol Microbiol 68, 3874 (2018); https://doi.org/10.1099/ijsem.0.003076
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