1887

Abstract

Two Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacteria, designated as SY3-15and SY3-13, were isolated from a seawater sample of the South China Sea. Colonies were 0.5–1.0 mm in diameter, smooth, circular, convex and translucent after growth on marine agar at 37 °C for 3 days. The strains were found to grow at 20–50 °C (optimum, 42 °C), pH 6.0–8.5 (optimum, pH 6.5–7.5) and with 0.5–6.0 % (w/v) NaCl (optimum, 1.5–2.0 %). Chemotaxonomic analysis showed the sole respiratory quinone to be ubiquinone-10, the major fatty acids (>10 %) were C16 : 0 3-OH, C19 : 0cyclo ω9c, C18 : 1 3-OH and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), and the polar lipids were phosphatidylglycerol, two unidentified aminolipids and three unidentified lipids. The DNA G+C content was 67.2–67.4 mol% calculated by genome. The 16S rRNA gene sequences of strains SY3-15 and SY3-13 were identical and related to the genus Lutibaculum with a similarity of 92.1 %. The 16S rRNA gene phylogenetic trees reconstructed with neighbour-joining, maximum-parsimony and minimum-evolution methods showed that the strains constituted a deep and separated branch from other families of Alphaproteobacteria , and the phylogenetic trees based on concatenated 163 protein sequences from genome sequences showed that the clade in which strains SY3-15 and SY3-13 located was separated from the clade of the other orders of Alphaproteobacteria , indicating it may represent a novel family of a novel order. Based on their phenotypic properties and their phylogenetic distinctiveness, we propose strains SY3-15 (=MCCC 1K03467=KCTC 62335) and SY3-13 (=MCCC 1K03466=KCTC 62329) to represent a novel species of a novel genus with the name Minwuia thermotolerans gen. nov., sp. nov., and we propose Minwuiaceae fam. nov. and Minwuiales ord. nov. with Minwuia as the type genus.

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2018-10-16
2024-12-03
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References

  1. Rosenberg E, DeLong FE, Lory S, Stackebrandt E, Thompson F et al. The Prokaryotes - Alphaproteobacteria and Betaproteobacteria, 4th ed. New York: Springer; 2014
    [Google Scholar]
  2. Parte AC. LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  3. Williams ST, Davies FL. Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. J Gen Microbiol 1965; 38:251–261 [View Article][PubMed]
    [Google Scholar]
  4. Zhang XQ, Wu YH, Zhou X, Zhang X, Xu XW et al. Parvularcula flava sp. nov., an alphaproteobacterium isolated from surface seawater of the South China Sea. Int J Syst Evol Microbiol 2016; 66:3498–3502 [View Article][PubMed]
    [Google Scholar]
  5. Pan J, Sun C, Zhang XQ, Huo YY, Zhu XF et al. Paracoccus sediminis sp. nov., isolated from Pacific Ocean marine sediment. Int J Syst Evol Microbiol 2014; 64:2512–2516 [View Article][PubMed]
    [Google Scholar]
  6. Su Y, Han S, Wang R, Yu X, Fu G et al. Microbaculum marinum gen. nov., sp. nov., isolated from deep seawater. Int J Syst Evol Microbiol 2017; 67:812–817 [View Article][PubMed]
    [Google Scholar]
  7. Leifson E. Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 1963; 85:1183–1184[PubMed]
    [Google Scholar]
  8. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  9. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207
    [Google Scholar]
  10. Sun C, Fu GY, Zhang CY, Hu J, Xu L et al. Isolation and complete genome sequence of Algibacter alginolytica sp. nov., a novel seaweed-degrading Bacteroidetes bacterium with diverse putative polysaccharide utilization loci. Appl Environ Microbiol 2016; 82:2975–2987 [View Article][PubMed]
    [Google Scholar]
  11. Jackman SD, Vandervalk BP, Mohamadi H, Chu J, Yeo S et al. ABySS 2.0: resource-efficient assembly of large genomes using a Bloom filter. Genome Res 2017; 27:768–777 [View Article][PubMed]
    [Google Scholar]
  12. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007; 35:3100–3108 [View Article][PubMed]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  16. Rzhetsky A, Nei M. A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 1992; 9:945–967
    [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  18. Aziz RK, Bartels D, Best AA, Dejongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008; 9:75 [View Article][PubMed]
    [Google Scholar]
  19. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
    [Google Scholar]
  20. Fischer S, Brunk BP, Chen F, Gao X, Harb OS et al. Using OrthoMCL to assign proteins to OrthoMCL-DB groups or to cluster proteomes into new ortholog groups. Curr Protoc Bioinformatics 2011; 12:1–19 [View Article][PubMed]
    [Google Scholar]
  21. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013; 30:772–780 [View Article][PubMed]
    [Google Scholar]
  22. Capella-Gutiérrez S, Silla-Martínez JM, Gabaldón T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 2009; 25:1972–1973 [View Article][PubMed]
    [Google Scholar]
  23. Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015; 32:268–274 [View Article][PubMed]
    [Google Scholar]
  24. Wang Z, Leary DH, Malanoski AP, Li RW, Hervey WJ et al. A previously uncharacterized, nonphotosynthetic member of the Chromatiaceae is the primary CO2-fixing constituent in a self-regenerating biocathode. Appl Environ Microbiol 2015; 81:699–712 [View Article][PubMed]
    [Google Scholar]
  25. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
    [Google Scholar]
  26. Siamphan C, Kim H, Lee JS, Kim W. Sneathiella chungangensis sp. nov., isolated from a marine sand, and emended description of the genus Sneathiella. Int J Syst Evol Microbiol 2014; 64:1468–1472 [View Article][PubMed]
    [Google Scholar]
  27. Kurahashi M, Fukunaga Y, Harayama S, Yokota A. Sneathiella glossodoripedis sp. nov., a marine alphaproteobacterium isolated from the nudibranch Glossodoris cincta, and proposal of Sneathiellales ord. nov. and Sneathiellaceae fam. nov. Int J Syst Evol Microbiol 2008; 58:548–552 [View Article][PubMed]
    [Google Scholar]
  28. Jordan EM, Thompson FL, Zhang XH, Li Y, Vancanneyt M et al. Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. Int J Syst Evol Microbiol 2007; 57:114–121 [View Article][PubMed]
    [Google Scholar]
  29. Balcázar JL, Planas M, Pintado J. Oceanibacterium hippocampi gen. nov., sp. nov., isolated from cutaneous mucus of wild seahorses (Hippocampus guttulatus). Antonie van Leeuwenhoek 2012; 102:187–191 [View Article][PubMed]
    [Google Scholar]
  30. Albuquerque L, Rainey FA, Nobre MF, da Costa MS. Oceanicella actignis gen. nov., sp. nov., a halophilic slightly thermophilic member of the Alphaproteobacteria. Syst Appl Microbiol 2012; 35:385–389 [View Article][PubMed]
    [Google Scholar]
  31. Yin D, Chen L, Ao J, Ai C, Chen X. Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring. Int J Syst Evol Microbiol 2013; 63:1868–1873 [View Article][PubMed]
    [Google Scholar]
  32. Albuquerque L, Rainey FA, Pena A, Tiago I, Veríssimo A et al. Tepidamorphus gemmatus gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria. Syst Appl Microbiol 2010; 33:60–66 [View Article][PubMed]
    [Google Scholar]
  33. Kumar PA, Srinivas TN, Manasa P, Madhu S, Shivaji S. Lutibaculum baratangense gen. nov., sp. nov., a proteobacterium isolated from a mud volcano. Int J Syst Evol Microbiol 2012; 62:2025–2031 [View Article][PubMed]
    [Google Scholar]
  34. Zhen-Li Z, Xin-Qi Z, Nan W, Wen-Wu Z, Xu-Fen Z et al. Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 2014; 64:2805–2811 [View Article][PubMed]
    [Google Scholar]
  35. Sun C, Wu C, Su Y, Wang RJ, Fu GY et al. Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater. Int J Syst Evol Microbiol 2017; 67:4024–4031 [View Article][PubMed]
    [Google Scholar]
  36. Sun C, Wang RJ, Su Y, Fu GY, Zhao Z et al. Hyphobacterium vulgare gen. nov., sp. nov., a novel alphaproteobacterium isolated from seawater. Int J Syst Evol Microbiol 2017; 67:1169–1176 [View Article][PubMed]
    [Google Scholar]
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