1887

Abstract

Four new limnic bacterial strains, namely HYN0048, HYN0049, HYN0056 and HYN0059, were isolated from two lakes in the Nakdong River water system in Korea. 16S rRNA gene sequence analyses indicated that strains HYN0048, HYN0049, HYN0056 and HYN0059 belonged to the genus Flavobacterium by showing the highest sequence similarities with the type strains of F. soli (94.9 %), F. keumense (95.1 %), F. dispersum (97.7 %) and F. hauense (97.6 %), respectively. The low sequence similarities and tree topologies implied the novelty of the four isolates, as novel genomic species of the genus Flavobacterium . Numerous physiological and biochemical features also supported the distinctiveness of the isolates from previously known bacterial species. On the basis of the phylogenetic and phenotypic data presented in this study, four novel species are suggested with the following names: Flavobacterium magnum sp. nov. (type strain HYN0048=KACC 19180=NBRC 112740), Flavobacterium pallidum sp. nov. (HYN0049=KACC 19181=NBRC 112741), Flavobacterium crocinum sp. nov. (HYN0056=KACC 19182=NBRC 112743) and Flavobacterium album sp. nov. (HYN0059=KACC 19183=NBRC 112744).

Keyword(s): Flavobacterium and lake water
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003067
2018-10-15
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/12/3837.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003067&mimeType=html&fmt=ahah

References

  1. Bergey DH, Harrison FC, Breed RS, Hb W, Huntoon FM et al. Genus II. Flavobacterium gen. nov. In Bergey's Manual of Determinative Bacteriology Baltimore: Williams and Wilkins; 1923; pp.97–117
    [Google Scholar]
  2. Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian Knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 1996;46:128–148 [CrossRef]
    [Google Scholar]
  3. Bernardet JF, Bowman JP. Genus I. Flavobacterium. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. USA: Springer; 2011; pp.112–154
    [Google Scholar]
  4. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  5. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014;64:689–691 [CrossRef][PubMed]
    [Google Scholar]
  6. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  7. Shin SK, Kim E, Choi S, Yi H. Cochleicola gelatinilyticus gen. nov., sp. nov., Isolated from a Marine Gastropod, Reichia luteostoma. J Microbiol Biotechnol 2016;26:1439–1445 [CrossRef][PubMed]
    [Google Scholar]
  8. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:1825 [CrossRef][PubMed]
    [Google Scholar]
  9. Shin SK, Ha Y, Cho YJ, Kwon S, Yong D et al. Flavobacterium gilvum sp. nov., isolated from stream water. Int J Syst Evol Microbiol 2016;67:153–157 [CrossRef][PubMed]
    [Google Scholar]
  10. Gerhardt P. Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994
    [Google Scholar]
  11. Ryu E. A simple method of staining bacterial flagella. Kitasato Archives of Experimental Medicine 1937;14:218–219
    [Google Scholar]
  12. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  13. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000;50:1861–1868 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003067
Loading
/content/journal/ijsem/10.1099/ijsem.0.003067
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error