1887

Abstract

Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 (99.9 % similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 and M. chelonae subsp. bovis KCTC 39630. Phylogenetic analysis based on whole genome sequences revealed a 95.89 % average nucleotide identity (ANI) value with M. chelonae subsp. chelonae , slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae . Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W (=KCTC 29127=JCM 32454).

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2018-10-12
2024-03-29
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References

  1. Runyon EH. Anonymous mycobacteria in pulmonary disease. Med Clin North Am 1959; 43:273–290 [View Article][PubMed]
    [Google Scholar]
  2. Petrini B. Mycobacterium abscessus: an emerging rapid-growing potential pathogen. APMIS 2006; 114:319–328 [View Article][PubMed]
    [Google Scholar]
  3. Bryant JM, Grogono DM, Greaves D, Foweraker J, Roddick I et al. Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. Lancet 2013; 381:1551–1560 [View Article][PubMed]
    [Google Scholar]
  4. Wallace RJ, Brown BA, Onyi GO. Skin, soft tissue, and bone infections due to Mycobacterium chelonae chelonae: importance of prior corticosteroid therapy, frequency of disseminated infections, and resistance to oral antimicrobials other than clarithromycin. J Infect Dis 1992; 166:405–412 [View Article][PubMed]
    [Google Scholar]
  5. Tortoli E, Kohl TA, Brown-Elliott BA, Trovato A, Leão SC et al. Emended description of Mycobacterium abscessus, Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. bolletii and designation of Mycobacterium abscessus subsp. massiliense comb. nov. Int J Syst Evol Microbiol 2016; 66:4471–4479 [View Article][PubMed]
    [Google Scholar]
  6. Kim BJ, Kim GN, Kim BR, Jeon CO, Jeong J et al. Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov. Int J Syst Evol Microbiol 2017; 67:3882–3887 [View Article][PubMed]
    [Google Scholar]
  7. Magee JG, Ward AC. Genus I. Mycobacterium. In Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Suzuki K et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 5 New York: Springer; 2012 pp. 312–375
    [Google Scholar]
  8. Hoefsloot W, van Ingen J, Andrejak C, Angeby K, Bauriaud R et al. The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study. Eur Respir J 2013; 42:1604–1613 [View Article][PubMed]
    [Google Scholar]
  9. Lee SY, Kim BJ, Kim H, Won YS, Jeon CO et al. Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare. Int J Syst Evol Microbiol 2016; 66:3132–3141 [View Article][PubMed]
    [Google Scholar]
  10. Kim BJ, Hong SH, Kook YH, Kim BJ. Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to Mycobacterium gordonae. Int J Syst Evol Microbiol 2014; 64:39–45 [View Article][PubMed]
    [Google Scholar]
  11. Kim BJ, Hong SH, Yu HK, Park YG, Jeong J et al. Mycobacterium parakoreense sp. nov., a slowly growing non-chromogenic species related to Mycobacterium koreense, isolated from a human clinical specimen. Int J Syst Evol Microbiol 2013; 63:2301–2308 [View Article][PubMed]
    [Google Scholar]
  12. Kim BJ, Math RK, Jeon CO, Yu HK, Park YG et al. Mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare. Int J Syst Evol Microbiol 2013; 63:192–199 [View Article][PubMed]
    [Google Scholar]
  13. Kim BJ, Jeong J, Lee SH, Kim SR, Yu HK, Hk Y et al. Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale. Int J Syst Evol Microbiol 2012; 62:1289–1295 [View Article][PubMed]
    [Google Scholar]
  14. Lee H, Lee SA, Lee IK, Yu HK, Park YG et al. Mycobacterium paraterrae sp. nov. recovered from a clinical specimen: novel chromogenic slow growing mycobacteria related to Mycobacterium terrae complex. Microbiol Immunol 2010; 54:46–53 [View Article][PubMed]
    [Google Scholar]
  15. Lee HK, Lee SA, Lee IK, Yu HK, Park YG et al. Mycobacterium paraseoulense sp. nov., a slowly growing, scotochromogenic species related genetically to Mycobacterium seoulense. Int J Syst Evol Microbiol 2010; 60:439–443 [View Article][PubMed]
    [Google Scholar]
  16. Mun HS, Park JH, Kim H, Yu HK, Park YG et al. Mycobacterium senuense sp. nov., a slowly growing, non-chromogenic species closely related to the Mycobacterium terrae complex. Int J Syst Evol Microbiol 2008; 58:641–646 [View Article][PubMed]
    [Google Scholar]
  17. Mun HS, Kim HJ, Oh EJ, Kim H, Bai GH et al. Mycobacterium seoulense sp. nov., a slowly growing scotochromogenic species. Int J Syst Evol Microbiol 2007; 57:594–599 [View Article][PubMed]
    [Google Scholar]
  18. Kim BJ, Kim JM, Kim BR, Lee SY, Kim G et al. Mycobacterium anyangense sp. nov., a rapidly growing species isolated from blood of Korean native cattle, Hanwoo (Bos taurus coreanae). Int J Syst Evol Microbiol 2015; 65:2277–2285 [View Article][PubMed]
    [Google Scholar]
  19. Kent PT, Kubica GP. Public Health Mycobacteriology: A Guide for the Level III Laboratory Centers for Disease Control and Prevention; 1985
    [Google Scholar]
  20. Pérez E, Constant P, Lemassu A, Laval F, Daffé M et al. Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J Biol Chem 2004; 279:42574–42583 [View Article][PubMed]
    [Google Scholar]
  21. Ripoll F, Deshayes C, Pasek S, Laval F, Beretti JL et al. Genomics of glycopeptidolipid biosynthesis in Mycobacterium abscessus and M. chelonae. BMC Genomics 2007; 8:114 [View Article][PubMed]
    [Google Scholar]
  22. Kim BJ, Yi SY, Shim TS, do SY, Yu HK et al. Discovery of a novel hsp65 genotype within Mycobacterium massiliense associated with the rough colony morphology. PLoS One 2012; 7:e38420 [View Article][PubMed]
    [Google Scholar]
  23. Butler WR, Thibert L, Kilburn JO. Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 1992; 30:2698–2704[PubMed]
    [Google Scholar]
  24. Butler WR, Guthertz LS. Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species. Clin Microbiol Rev 2001; 14:704–726 [View Article][PubMed]
    [Google Scholar]
  25. Kim H, Kim SH, Shim TS, Kim MN, Bai GH et al. Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int J Syst Evol Microbiol 2005; 55:1649–1656 [View Article][PubMed]
    [Google Scholar]
  26. Springer B, Stockman L, Teschner K, Roberts GD, Böttger EC. Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 1996; 34:296–303[PubMed]
    [Google Scholar]
  27. Macheras E, Roux AL, Bastian S, Leão SC, Palaci M et al. Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains. J Clin Microbiol 2011; 49:491–499 [View Article][PubMed]
    [Google Scholar]
  28. Macheras E, Konjek J, Roux AL, Thiberge JM, Bastian S et al. Multilocus sequence typing scheme for the Mycobacterium abscessus complex. Res Microbiol 2014; 165:82–90 [View Article][PubMed]
    [Google Scholar]
  29. Jukes TH, Cantor CR. Mammalian Protein Metabolism New York: Academic Press; 1969
    [Google Scholar]
  30. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  31. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  32. Kumar S, Nei M, Dudley J, Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 2008; 9:299–306 [View Article][PubMed]
    [Google Scholar]
  33. Fukano H, Wada S, Kurata O, Katayama K, Fujiwara N et al. Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer. Int J Syst Evol Microbiol 2017; 67:2811–2817 [View Article][PubMed]
    [Google Scholar]
  34. Fukano H, Yoshida M, Katayama Y, Omatsu T, Mizutani T et al. Complete genome sequence of Mycobacterium stephanolepidis. Genome Announc 2017; 5:e00810-17 [View Article][PubMed]
    [Google Scholar]
  35. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  36. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
    [Google Scholar]
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