1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic and orange-pigmented marine bacterium, designated strain L53, was isolated from an oyster sample collected from the coast of Weihai, China (122.0° E 37.5° N). Growth of strain L53 occurred at 4–40 °C (optimum, 33 °C), at pH 6.5–8.0 (optimum, pH 7.5) and with 1.0–7.0 % (w/v) NaCl (optimum, 3.0 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain L53 was closely related to Bizionia echini KCTC 22015 (96.9 %) and Hanstruepera neustonica JCM 19743 (96.1 %). Strain L53 was located in a distinct phyletic lineage in a discrete clade associated with H. neustonica JCM 19743. The DNA G+C content of strain L53 was 33.5 mol%. The sole menaquinone was MK-6. The polar lipids comprised one phosphatidylethanolamine, four unidentified aminolipids and four unidentified lipids. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 G. Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain L53 should be placed in the genus Hanstruepera as representing a novel species, for which the name Hanstruepera crassostreae sp. nov. is proposed. The type strain is L53 (=KCTC 62247=MCCC 1H00246).

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2018-10-10
2019-10-22
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References

  1. Hameed A, Shahina M, Lai WA, Lin SY, Liu YC et al. Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014. Int J Syst Evol Microbiol 2015;65:336–345 [CrossRef][PubMed]
    [Google Scholar]
  2. Williams GP, Babu S, Ravikumar S, Kathiresan K, Prathap SA et al. Antimicrobial activity of tissue and associated bacteria from benthic sea anemone Stichodactyla haddoni against microbial pathogens. J Environ Biol 2007;28:789–793[PubMed]
    [Google Scholar]
  3. Wang X, Huang Y, Sheng Y, Su P, Qiu Y et al. Antifouling activity towards mussel by small-molecule compounds from a strain of Vibrio alginolyticus bacterium associated with sea anemone Haliplanella sp. J Microbiol Biotechnol 2017;27:460–470 [CrossRef][PubMed]
    [Google Scholar]
  4. Indraningrat AA, Smidt H, Sipkema D. Bioprospecting sponge-associated microbes for antimicrobial compounds. Mar Drugs 2016;14:87 [CrossRef][PubMed]
    [Google Scholar]
  5. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014;64:2204–2209 [CrossRef][PubMed]
    [Google Scholar]
  6. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  9. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971;20:406–416 [CrossRef]
    [Google Scholar]
  10. Besemer J, Lomsadze A, Borodovsky M. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res 2001;29:2607–2618 [CrossRef][PubMed]
    [Google Scholar]
  11. Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 1997;25:955–964[PubMed]
    [Google Scholar]
  12. Kanehisa M, Goto S, Kawashima S, Okuno Y, Hattori M. The KEGG resource for deciphering the genome. Nucleic Acids Res 2004;32:277D–280 [CrossRef][PubMed]
    [Google Scholar]
  13. Medema MH, Blin K, Cimermancic P, de Jager V, Zakrzewski P et al. antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences. Nucleic Acids Res 2011;39:W339–W346 [CrossRef][PubMed]
    [Google Scholar]
  14. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef][PubMed]
    [Google Scholar]
  15. Rodriguezr LM, Konstantinidis KT. The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes. Peer J Prepr 2016;4:e1900v1
    [Google Scholar]
  16. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110:1281–1286 [CrossRef][PubMed]
    [Google Scholar]
  17. Smibert RM, Krieg NR. Phenotypic characterization. In Gerbardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Sociey for Microbiology; 1994; pp.607–654
    [Google Scholar]
  18. Xia HF, Li XL, Liu QQ, Miao TT, Du ZJ et al. Salegentibacter echinorum sp. nov., isolated from the sea urchin Hemicentrotus pulcherrimus. Antonie van Leeuwenhoek 2013;104:315–320 [CrossRef][PubMed]
    [Google Scholar]
  19. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  20. Hameed A, Shahina M, Lin SY, Sridhar KR, Young LS et al. Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan. FEMS Microbiol Lett 2012;333:37–45 [CrossRef][PubMed]
    [Google Scholar]
  21. Dong XZ, Cai MY. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  22. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014;64:1690–1696 [CrossRef][PubMed]
    [Google Scholar]
  23. CLSI Performance Standards for Antimicrobial Susceptibility Testing, 28th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2018
    [Google Scholar]
  24. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996;42:457–469 [CrossRef]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: 1990
    [Google Scholar]
  26. Tindall B, Sikorski J, Smibert R, Krieg N. Phenotypic characterization and the principles of comparative systematics. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T et al. (editors) Methods for General and Molecular Microbiology Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  28. Nedashkovskaya OI, Vancanneyt M, Kim SB. Bizionia echini sp. nov., isolated from a sea urchin. Int J Syst Evol Microbiol 2010;60:928–931 [CrossRef][PubMed]
    [Google Scholar]
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