1887

Abstract

A new actinobacterial strain, LCR6-01, was isolated from a lichen sample collected from Chiang Rai Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Streptomyces . The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0, iso-C14 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified lipid. The genomic DNA G+C content was 74.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the strain belonged to the genus Streptomyces . The strain showed highest 16S rRNA gene sequence similarity to Streptomyces palmae TBRC 1999 (98.6 %), Streptomyces misionensis JCM 4497 (98.6 %), Streptomyces matensis JCM 4268 (98.5 %), Streptomyces althioticus KCTC 9752 (98.5 %) and Streptomyces wuyuanensis KCTC 29112 (98.4 %). DNA–DNA relatedness values among the strain and closely related Streptomyces species were below 70 %. On the basis of the phenotypic characteristics and genotypic distinctiveness, strain LCR6-01 is considered to represent a novel species of the genus Streptomyces , for which the name Streptomyces lichenis sp. nov. is proposed. The type strain is LCR6-01 (=KCTC 39908=TISTR 2500)

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2018-10-10
2019-12-15
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