1887

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Volume 68, Issue 11

Pseudoruegeria insulae sp. nov., isolated from a tidal flat Free

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20, was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20 fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20 exhibited 16S rRNA gene sequence similarity values of 97.4–98.3 % to the type strains of Pseudoruegeria haliotis , Pseudoruegeria lutimaris , ‘ Pseudoruegeria litorisediminis’ and Pseudoruegeria sabulilitoris and 96.4–96.9 % to the type strains of the other Pseudoruegeria species. Strain BPTF-M20 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain BPTF-M20 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20 was 63.2 mol%. Mean DNA–DNA relatedness values of strain BPTF-M20 with the type strains of P. haliotis , P. lutimaris , P. sabulilitoris and ‘P. litorisediminis’ were 18–27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20 was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20 is considered to represent a novel species of the genus Pseudoruegeria , for which the name Pseudoruegeria insulae sp. nov. is proposed. The type strain is BPTF-M20 (=KACC 19614=KCTC 62422=NBRC 113188).

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Keyword(s): novel species , polyphasic taxonomy , Pseudoruegeria insulae and tidal flat
© 2018 IUMS
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2018-09-21
2024-04-24
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Pseudoruegeria insulae sp. nov., isolated from a tidal flat
Int J Syst Evol Microbiol 68, 3587 (2018); https://doi.org/10.1099/ijsem.0.003035
/content/journal/ijsem/10.1099/ijsem.0.003035
/content/journal/ijsem/10.1099/ijsem.0.003035
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