1887

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20, was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20 fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20 exhibited 16S rRNA gene sequence similarity values of 97.4–98.3 % to the type strains of Pseudoruegeria haliotis , Pseudoruegeria lutimaris , ‘ Pseudoruegeria litorisediminis’ and Pseudoruegeria sabulilitoris and 96.4–96.9 % to the type strains of the other Pseudoruegeria species. Strain BPTF-M20 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain BPTF-M20 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20 was 63.2 mol%. Mean DNA–DNA relatedness values of strain BPTF-M20 with the type strains of P. haliotis , P. lutimaris , P. sabulilitoris and ‘P. litorisediminis’ were 18–27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20 was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20 is considered to represent a novel species of the genus Pseudoruegeria , for which the name Pseudoruegeria insulae sp. nov. is proposed. The type strain is BPTF-M20 (=KACC 19614=KCTC 62422=NBRC 113188).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003035
2018-09-21
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/11/3587.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003035&mimeType=html&fmt=ahah

References

  1. Yoon JH, Lee SY, Kang SJ, Lee CH, Oh TK. Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 2007;57:542–547 [CrossRef][PubMed]
    [Google Scholar]
  2. Parte AC. LPSN - list of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018;68:1825–1829 [CrossRef][PubMed]
    [Google Scholar]
  3. Jung YT, Kim BH, Oh TK, Yoon JH. Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria. Int J Syst Evol Microbiol 2010;60:1177–1181 [CrossRef][PubMed]
    [Google Scholar]
  4. Hyun DW, Shin NR, Kim MS, Kim PS, Kim JY et al. Pseudoruegeria haliotis sp. nov., isolated from the gut of the abalone Haliotis discus hannai. Int J Syst Evol Microbiol 2013;63:4626–4632 [CrossRef][PubMed]
    [Google Scholar]
  5. Park S, Jung YT, Won SM, Yoon JH. Pseudoruegeria sabulilitoris sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 2014;64:3276–3281 [CrossRef][PubMed]
    [Google Scholar]
  6. Cha IT, Park I, Lee HW, Lee H, Park JM et al. Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat. Int J Syst Evol Microbiol 2016;66:3125–3131 [CrossRef][PubMed]
    [Google Scholar]
  7. Zhang Y, Xu Y, Fang W, Wang X, Fang Z et al. Pseudoruegeria marinistellae sp. nov., isolated from an unidentified starfish in Sanya, China. Antonie van Leeuwenhoek 2017;110:187–194 [CrossRef][PubMed]
    [Google Scholar]
  8. Park S, Park JM, Lee JS, Oh TK, Yoon JH. Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat. Arch Microbiol 2018;200:1183–1189 [CrossRef][PubMed]
    [Google Scholar]
  9. Yoon J-H, Kim H, Kim S-B, Kim H-J, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996;46:502–505 [CrossRef]
    [Google Scholar]
  10. Yoon J-H, Lee ST, Kim S-B, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997;47:111–114 [CrossRef]
    [Google Scholar]
  11. Yoon JH, Kang KH, Park YH. Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2003;53:449–454 [CrossRef][PubMed]
    [Google Scholar]
  12. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  13. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207
    [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  16. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994; pp.121–161
    [Google Scholar]
  17. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  18. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014;64:2969–2974 [CrossRef][PubMed]
    [Google Scholar]
  19. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Mocrobiol 1987;19:1–67
    [Google Scholar]
  20. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001;51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  21. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993
    [Google Scholar]
  22. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes Berlin: Springer; 1981; pp.1302–1331
    [Google Scholar]
  23. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957;49:25–68 [CrossRef][PubMed]
    [Google Scholar]
  24. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968;95:1921–1942[PubMed]
    [Google Scholar]
  25. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  26. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003035
Loading
/content/journal/ijsem/10.1099/ijsem.0.003035
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error