1887

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20, was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20 fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20 exhibited 16S rRNA gene sequence similarity values of 97.4–98.3 % to the type strains of Pseudoruegeria haliotis , Pseudoruegeria lutimaris , ‘ Pseudoruegeria litorisediminis’ and Pseudoruegeria sabulilitoris and 96.4–96.9 % to the type strains of the other Pseudoruegeria species. Strain BPTF-M20 contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain BPTF-M20 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20 was 63.2 mol%. Mean DNA–DNA relatedness values of strain BPTF-M20 with the type strains of P. haliotis , P. lutimaris , P. sabulilitoris and ‘P. litorisediminis’ were 18–27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20 was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20 is considered to represent a novel species of the genus Pseudoruegeria , for which the name Pseudoruegeria insulae sp. nov. is proposed. The type strain is BPTF-M20 (=KACC 19614=KCTC 62422=NBRC 113188).

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2018-09-21
2024-11-07
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References

  1. Yoon JH, Lee SY, Kang SJ, Lee CH, Oh TK. Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 2007; 57:542–547 [View Article][PubMed]
    [Google Scholar]
  2. Parte AC. LPSN - list of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 2018; 68:1825–1829 [View Article][PubMed]
    [Google Scholar]
  3. Jung YT, Kim BH, Oh TK, Yoon JH. Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria. Int J Syst Evol Microbiol 2010; 60:1177–1181 [View Article][PubMed]
    [Google Scholar]
  4. Hyun DW, Shin NR, Kim MS, Kim PS, Kim JY et al. Pseudoruegeria haliotis sp. nov., isolated from the gut of the abalone Haliotis discus hannai. Int J Syst Evol Microbiol 2013; 63:4626–4632 [View Article][PubMed]
    [Google Scholar]
  5. Park S, Jung YT, Won SM, Yoon JH. Pseudoruegeria sabulilitoris sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 2014; 64:3276–3281 [View Article][PubMed]
    [Google Scholar]
  6. Cha IT, Park I, Lee HW, Lee H, Park JM et al. Pseudoruegeria aestuarii sp. nov., of the family Rhodobacteraceae, isolated from a tidal flat. Int J Syst Evol Microbiol 2016; 66:3125–3131 [View Article][PubMed]
    [Google Scholar]
  7. Zhang Y, Xu Y, Fang W, Wang X, Fang Z et al. Pseudoruegeria marinistellae sp. nov., isolated from an unidentified starfish in Sanya, China. Antonie van Leeuwenhoek 2017; 110:187–194 [View Article][PubMed]
    [Google Scholar]
  8. Park S, Park JM, Lee JS, Oh TK, Yoon JH. Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat. Arch Microbiol 2018; 200:1183–1189 [View Article][PubMed]
    [Google Scholar]
  9. Yoon J-H, Kim H, Kim S-B, Kim H-J, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46:502–505 [View Article]
    [Google Scholar]
  10. Yoon J-H, Lee ST, Kim S-B, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47:111–114 [View Article]
    [Google Scholar]
  11. Yoon JH, Kang KH, Park YH. Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2003; 53:449–454 [View Article][PubMed]
    [Google Scholar]
  12. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  13. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207
    [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  16. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994 pp. 121–161
    [Google Scholar]
  17. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  18. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:2969–2974 [View Article][PubMed]
    [Google Scholar]
  19. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Mocrobiol 1987; 19:1–67
    [Google Scholar]
  20. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  21. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993
    [Google Scholar]
  22. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes Berlin: Springer; 1981 pp. 1302–1331
    [Google Scholar]
  23. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957; 49:25–68 [View Article][PubMed]
    [Google Scholar]
  24. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968; 95:1921–1942[PubMed]
    [Google Scholar]
  25. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
    [Google Scholar]
  26. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
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